Molecular characterization and phylogenetic analysis of Trypanosoma spp. detected from striped leaf-nosed bats (Hipposideros vittatus) in Zambia

Yongjin Qiu; Masahiro Kajihara; Hayato Harima; Bernard Mudenda Hang'ombe; Ryo Nakao; Kyoko Hayashida; Akina Mori-Kajihara; Katendi Changula; Yoshiki Eto; Joseph Ndebe; Reiko Yoshida; Yoshihiro Takadate; Daniel Mwizabi; Hiroki Kawabata; Martin Simuunza; Aaron Mweene; Hirofumi Sawa; Ayato Takada; Chihiro Sugimoto

International Journal for Parasitology: Parasites and Wildlife (Aug 2019)

Molecular characterization and phylogenetic analysis of Trypanosoma spp. detected from striped leaf-nosed bats (Hipposideros vittatus) in Zambia

Yongjin Qiu,
Masahiro Kajihara,
Hayato Harima,
Bernard Mudenda Hang'ombe,
Ryo Nakao,
Kyoko Hayashida,
Akina Mori-Kajihara,
Katendi Changula,
Yoshiki Eto,
Joseph Ndebe,
Reiko Yoshida,
Yoshihiro Takadate,
Daniel Mwizabi,
Hiroki Kawabata,
Martin Simuunza,
Aaron Mweene,
Hirofumi Sawa,
Ayato Takada,
Chihiro Sugimoto

Affiliations

Yongjin Qiu: Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, The University of Zambia, PO Box 32379, Lusaka, 10101, Zambia
Masahiro Kajihara: Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan
Hayato Harima: Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, The University of Zambia, PO Box 32379, Lusaka, 10101, Zambia
Bernard Mudenda Hang'ombe: Department of Para-clinical Studies, School of Veterinary Medicine, The University of Zambia, P.O. Box 32379, Lusaka, 10101, Zambia; Global Virus Network Affilate Center of Excellence, The University of Zambia, P.O. Box 32379, Lusaka, 10101, Zambia; African Center of Excellence for Infectious Diseases of Humans and Animals, P.O. Box 32379, Lusaka, 10101, Zambia
Ryo Nakao: Laboratory of Veterinary Parasitology, Graduate School of Infectious Diseases, Hokkaido University, Kita-18 Nishi-9, Kita-ku, Sapporo, Hokkaido, 060-0818, Japan
Kyoko Hayashida: Division of Collaboration and Education, Hokkaido University Research Center for Zoonosis Control, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan
Akina Mori-Kajihara: Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan
Katendi Changula: Department of Para-clinical Studies, School of Veterinary Medicine, The University of Zambia, P.O. Box 32379, Lusaka, 10101, Zambia
Yoshiki Eto: Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan
Joseph Ndebe: Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, 10101, Zambia
Reiko Yoshida: Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan
Yoshihiro Takadate: Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan
Daniel Mwizabi: Department of National Parks and Wildlife, Ministry of Tourism and Arts, Chilanga, 101010, Zambia
Hiroki Kawabata: Department of Bacteriology-I, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjyuku-ku, Tokyo, 162-8640, Japan
Martin Simuunza: Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, 10101, Zambia
Aaron Mweene: Global Virus Network Affilate Center of Excellence, The University of Zambia, P.O. Box 32379, Lusaka, 10101, Zambia; African Center of Excellence for Infectious Diseases of Humans and Animals, P.O. Box 32379, Lusaka, 10101, Zambia; Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, 10101, Zambia
Hirofumi Sawa: Global Virus Network Affilate Center of Excellence, The University of Zambia, P.O. Box 32379, Lusaka, 10101, Zambia; African Center of Excellence for Infectious Diseases of Humans and Animals, P.O. Box 32379, Lusaka, 10101, Zambia; Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, 10101, Zambia; Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan; Global Virus Network, 725 West Lombard St, Baltimore, MD, 21201, USA; Division of Molecular Pathology, Hokkaido University Research Center for Zoonosis Control, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan
Ayato Takada: Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan; Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, 10101, Zambia; Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan
Chihiro Sugimoto: Division of Collaboration and Education, Hokkaido University Research Center for Zoonosis Control, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan; Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka, 10101, Zambia; Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Kita-20 Nishi-10, Kita-ku, Sapporo, Hokkaido, 001-0020, Japan; Corresponding author. Division of Collaboration and Education, Hokkaido University Research Center for Zoonosis Control, Kita 20, Nishi 10, Kita-Ku, Sapporo, Hokkaido, 001-0020, Japan.

Journal volume & issue: Vol. 9
pp. 234 – 238

Abstract

Read online

Bat trypanosomes consist of more than 30 trypanosome species from over 70 species of bats. Recent studies suggest that bats play a role in disseminating trypanosomes from African continent to the terrestrial mammals both in the Afrotropic-Palearctic Ecozones and Nearctic Ecozone. However, the diversity, distribution, and evolution of bat trypanosomes are still unclear. To better understand their evolution, more genetic data of bat trypanosomes from a variety of locations are required. During a survey of Borrelia spp. of bats inhabiting a cave in Zambia, we observed flagellate parasites from 5 of 43 hemocultures. Sequence and phylogenetic analyses of the glycosomal glyceraldehyde 3-phosphate dehydrogenase gene (gGAPDH; 572 bp) and the 18S ribosomal RNA gene (18S rRNA gene; 1,079–1,091 bp) revealed that all were Trypanosoma spp. belonged to the Trypanosoma cruzi clade. Three and two of them exhibited the similarity with T. conorhini and T. dionisii, respectively. The present study provides the first genetic data on Trypanosoma spp. of bats inhabiting Zambia. Keywords: Zambia, Bat, Trypanosoma cruzi clade, Trypanosoma conorhini, Trypanosoma dionisii

Published in International Journal for Parasitology: Parasites and Wildlife

ISSN: 2213-2244 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Zoology
Website: http://www.journals.elsevier.com/international-journal-for-parasitology-parasites-and-wildlife/

About the journal