Purification of Native Acetyl CoA Carboxylase From Mammalian Cells
Yaxue Sun,
Jiachen Li,
Lianmei Zhao,
Hongtao Zhu
Affiliations
Yaxue Sun
Hebei Provincial Key Laboratory of Tumour Prevention and Precision Diagnosis and Treatment, Shijiazhuang, Hebei, ChinaMedical School, He'bei University, Shijiazhuang, Hebei, China, Beijing National Laboratory for Condensed Matter Physics and Laboratory of Soft Matter Physics, Institute of Physics, Beijing, China
Jiachen Li
Hebei Provincial Key Laboratory of Tumour Prevention and Precision Diagnosis and Treatment, Shijiazhuang, Hebei, ChinaMedical School, He'bei University, Shijiazhuang, Hebei, China, Beijing National Laboratory for Condensed Matter Physics and Laboratory of Soft Matter Physics, Institute of Physics, Beijing, China
Lianmei Zhao
Hebei Provincial Key Laboratory of Tumour Prevention and Precision Diagnosis and Treatment, Shijiazhuang, Hebei, ChinaMedical School, He'bei University, Shijiazhuang, Hebei, China
Hongtao Zhu
Beijing National Laboratory for Condensed Matter Physics and Laboratory of Soft Matter Physics, Institute of Physics, Beijing, ChinaUniversity of Chinese Academy of Sciences, Beijing, China
Fatty acid (FA) biosynthesis is a crucial cellular process that converts nutrients into metabolic intermediates necessary for membrane biosynthesis, energy storage, and the production of signaling molecules. Acetyl-CoA carboxylase (ACACA) plays a pivotal catalytic role in both fatty acid synthesis and oxidation. This cytosolic enzyme catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, which represents the first and rate-limiting step in de novo fatty acid biosynthesis. In this study, we developed a rapid and effective purification scheme for separating human ACACA without any exogenous affinity tags, providing researchers with a novel method to obtain human ACACA in its native form.
