The EuroBiotech Journal (Jul 2021)

Comparison of RT-qPCR results of different gene targets for SARS-CoV-2 in asymptomatic individuals during COVID-19 pandemic

  • Tuncel Gulten,
  • Ergoren Mahmut Cerkez,
  • Baddal Buket,
  • Tulay Pinar,
  • Arikan Ayse,
  • Guler Emrah,
  • Ozverel Cenk Serhan,
  • Kaya Suer H.,
  • Sayan Murat,
  • Sanlidag Tamer,

DOI
https://doi.org/10.2478/ebtj-2021-0018
Journal volume & issue
Vol. 5, no. s1
pp. 26 – 31

Abstract

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A reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is regarded as the most sensitive method available and is being used for screening procedure for all incoming passengers to Northern Cyprus for SARS-CoV-2. This study investigated the compatibility of two different RT-qPCR methodologies Diagnovital® and Bio-Speedy® by re-analyzing the previously confirmed positive samples. A total of 43 previously confirmed positive samples were re-analyzed by two different commercially available SARS-CoV-2 RT-qPCR kits. Only 23.5% of positive samples detected by Diagnovital® RT-qPCR kit were detected by Bio-Speedy® detection kit.

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