DNA PANEL DESIGN FOR GENOMIC STUDIES IN RUSSIAN CATTLE BREEDS

N. S. Yudin; L. A. Vasil’eva; V. A. Belyavskaya; R. B. Aitnazarov; P. N. Smirnov; M. Heaton; W. W. Laegreid; G. V. Orlova; A. G. Romashchenko; M. I. Voevoda

Вавиловский журнал генетики и селекции (Jan 2015)

DNA PANEL DESIGN FOR GENOMIC STUDIES IN RUSSIAN CATTLE BREEDS

N. S. Yudin,
L. A. Vasil’eva,
V. A. Belyavskaya,
R. B. Aitnazarov,
P. N. Smirnov,
M. Heaton,
W. W. Laegreid,
G. V. Orlova,
A. G. Romashchenko,
M. I. Voevoda

Affiliations

N. S. Yudin: Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia Institute of Internal Medicine, Novosibirsk, Russia Novosibirsk National Research State University, Novosibirsk, Russia
L. A. Vasil’eva: Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia
V. A. Belyavskaya: State Research Center of Virology and Biotechnology VECTOR, Novosibirsk, Russia
R. B. Aitnazarov: Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia
P. N. Smirnov: Novosibirsk State Agricultural University, Novosibirsk, Russia
M. Heaton: Meat Animal Research Center, United States Department of Agriculture, Clay Center, USA
W. W. Laegreid: Wyoming State Veterinary Laboratory, Department of Veterinary Sciences, University of Wyoming, Laramie, USA
G. V. Orlova: Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia
A. G. Romashchenko: Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia
M. I. Voevoda: Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia Institute of Internal Medicine, Novosibirsk, Russia Novosibirsk National Research State University, Novosibirsk, Russia

Journal volume & issue: Vol. 18, no. 3
pp. 463 – 468

Abstract

Read online

A panel of 96 DNA samples that reflects the breadth of genetic diversity in popular Russian cattle breeds has been designed. The panel of cattle DNA contains 11 dairy breeds and 6 beef and beef-dairy breeds. The numbers of animals in each breed group vary from 4 to 8. The main criterion for selection of individual animals within each breed is to maximize the total number of unshared haploid genomes according to pedigree data. The resulting panel is equivalent to USDA MARC Beef Cattle Diversity Panel version 2.1 in the power of SNP identification (number of unshared haploid genomes = 186,1, minimum allele frequency required for its detection with 95 % probability – 1,6 %). Analysis of three SNPs shows an insignificant difference between the allele frequencies in Galloway, Hereford, Grey Ukrainian, and Black Pied herds and those in the panel. Thus, the diversity panel may be useful for identification of genetic markers associated with economically important traits in cattle, evaluation of purebred and crossbred animals, and, probably, for tentative estimation of allele frequencies in commercial populations.

Published in Вавиловский журнал генетики и селекции

ISSN: 2500-0462 (Print); 2500-3259 (Online)
Publisher: Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
Country of publisher: Russian Federation
LCC subjects: Science: Biology (General): Genetics
Website: http://vavilov.elpub.ru/

About the journal

Abstract

Keywords