Expression of GADD34 and GSDD45 mRNA in U87 glioma cells: effect of ERN1 signaling enzyme and glucose deprivation

Abstract

GADD34 and GADD45 factors play an important role in suppression of malignant tumor growth. It was shown that blockade of both enzymatic functions of sensor and signaling enzyme ERN1 (from endoplasmic reticulum to nuclei-1), the major component of endoplasmic reticulum stress signaling, decreases the expression level of genes GADD34 and GADD45A and increases the GADD45B gene expression in U87 glioma cell. The increase of GADD45B and decrease of GADD34 and GADD45A gene expressions in glioma cells with ERN1 signaling enzyme loss of function is possibly participated in suppression of these cells proliferation. It was shown that, that glucose deprivation condition leads to enhance the expression of GADD34, GADD45A and GADD45В genes in both types of glioma cells, but complete blockade of ERN1 enzyme function modifies the effect of glucose deprivation condition on the expression level all of these genes. Thus, results of this investigation demonstrated that the expression of GADD34, GADD45A and GADD45В genes in U87 glioma cells is dependent from signaling enzyme ERN1 and is changed by glucose deprivation condition, but its effect depends from ERN1 signaling enzyme function.

Keywords

• GADD34, GADD45, glucose, glioma cells