Association of Tissue Transglutaminase with Nerve Growth Factor, Prolidase Activity and Oxidative Stress in Celiac Patients

Abstract

Introduction: Celiac Disease (CD) is an autoimmune gastrointestinal disorder; it is characterised by chronic inflammation and elevation of tissue Transglutaminase Immunoglobulin-A (tTG-IgA). Nerve Growth Factor (NGF) and prolidase enzyme are elevated in different autoimmune and chronic inflammatory diseases; however, the status of NGF and prolidase enzyme remains unexplored in celiac patients till date. Aim: To evaluate the correlation of tTG-IgA to NGF, Serum Prolidase Activity (SPA) and oxidative stress in the CD patients. Materials and Methods: The study was conducted on a total of 92 subjects, 46 patients with CD and remaining 46 healthy controls. The tTG-IgA and NGF were measured with the use of ELISA kit. SPA and oxidative stress were measured spectrophotometrically. Results: The tTG-IgA, NGF, SPA, Total Oxidant Status (TOS) and Oxidative Stress Index (OSI) were significantly elevated in the serum of patients as compared to controls (all p<0.001); however, Total Antioxidant Status (TAS) was significantly decreased (p<0.001). The tTG-IgA showed a positive correlation with NGF, SPA, TOS and OSI in the patients (all p<0.001); while it was negatively correlated to TAS (p<0.001). Conclusion: The present study concluded that elevated NGF, prolidase enzyme, tTG and oxidative stress might be associated with the pathogenesis of celiac disease.

Keywords

• prolidase enzyme
• total antioxidant status
• total oxidant status