A whole-mount immunohistochemistry protocol for detection of mouse corneal nerves

Abstract

Summary: A cornea is innervated by sensory nerves, which branch into thick trunks, subbasal plexuses, and sensory endings. Appropriate assessment of nerve structure in a tissue provides a more complete understanding of the role of nerves in health and disease. Here, we present a whole-mount immunohistochemistry protocol that facilitates evaluation of nerve architecture throughout the mouse cornea. The fixation step in this protocol allows for reliable detection of nerve structures within the cornea and likely other tissues.For complete details on the use and execution of this protocol, please refer to Yun et al, (2020).

Keywords

• Microscopy
• Neuroscience