Toxins (Aug 2023)

Deciphering the Hazardous Effects of AFB1 and T-2 Toxins: Unveiling Toxicity and Oxidative Stress Mechanisms in PK15 Cells and Mouse Kidneys

  • Shuai Xiao,
  • Yingxin Wu,
  • Suisui Gao,
  • Mingxia Zhou,
  • Zhiwei Liu,
  • Qianbo Xiong,
  • Lihuang Jiang,
  • Guoxiang Yuan,
  • Linfeng Li,
  • Lingchen Yang

DOI
https://doi.org/10.3390/toxins15080503
Journal volume & issue
Vol. 15, no. 8
p. 503

Abstract

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In China, animal feeds are frequently contaminated with a range of mycotoxins, with Aflatoxin B1 (AFB1) and T-2 toxin (T-2) being two highly toxic mycotoxins. This study investigates the combined nephrotoxicity of AFB1 and T-2 on PK15 cells and murine renal tissues and their related oxidative stress mechanisms. PK15 cells were treated with the respective toxin concentrations for 24 h, and oxidative stress-related indicators were assessed. The results showed that the combination of AFB1 and T-2 led to more severe cellular damage and oxidative stress compared to exposure to the individual toxins (p p < 0.05). These findings suggest that the combined toxins cause significant oxidative damage to mouse kidneys. The study highlights the importance of considering the combined effects of mycotoxins in animal feed, particularly AFB1 and T-2, which can lead to severe nephrotoxicity and oxidative stress in PK15 cells and mouse kidneys. The findings have important implications for animal feed safety and regulatory policy.

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