DNA Barcoding Is a Useful Tool for the Identification of the Family <i>Scaridae</i> in Hainan

Bo Liu; Yali Yan; Nan Zhang; Huayang Guo; Baosuo Liu; Jingwen Yang; Kecheng Zhu; Dianchang Zhang

doi:10.3390/jmse10121915

Journal of Marine Science and Engineering (Dec 2022)

DNA Barcoding Is a Useful Tool for the Identification of the Family <i>Scaridae</i> in Hainan

Bo Liu,
Yali Yan,
Nan Zhang,
Huayang Guo,
Baosuo Liu,
Jingwen Yang,
Kecheng Zhu,
Dianchang Zhang

Affiliations

Bo Liu: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China
Yali Yan: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China
Nan Zhang: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China
Huayang Guo: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China
Baosuo Liu: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China
Jingwen Yang: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China
Kecheng Zhu: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China
Dianchang Zhang: South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China

DOI: https://doi.org/10.3390/jmse10121915
Journal volume & issue: Vol. 10, no. 12
p. 1915

Abstract

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Species markers can be quickly and accurately assessed using DNA barcoding. We investigated samples from the parrotfish family Scaridae using DNA barcoding in Hainan. A total of 401 DNA barcodes were analyzed, including 51 new barcodes generated from fresh material, based on a 533 bp fragment of the cytochrome c oxidase subunit I (CO I) gene. There were 350 CO I barcode clusters that matched 43 species from the Barcode of Life Data Systems (BOLD) and GenBank databases. The results showed the following average nucleotide compositions for the complete dataset: adenine (A, 22.7%), thymine (T, 29.5%), cytosine (C, 29.5%), and guanine (G, 18.2%). The mean genetic distance between confamilial species was nearly 53-fold greater than that between individuals within the species. In the neighbor-joining tree of CO I sequences, Chlorurus sordidus and C. spilurus clustered together, and all other individuals clustered by species. Our results indicated that DNA barcoding could be used as an effective molecular tool for monitoring, protecting, and managing fisheries, and for elucidating taxonomic problem areas that require further investigation.

Published in Journal of Marine Science and Engineering

ISSN: 2077-1312 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Naval Science: Naval architecture. Shipbuilding. Marine engineering; Geography. Anthropology. Recreation: Oceanography
Website: http://www.mdpi.com/journal/jmse

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