<i>Aronia melanocarpa</i> Extract Fermented by <i>Lactobacillus plantarum</i> EJ2014 Modulates Immune Response in Mice
Md. Sekendar Ali,
Eon-Bee Lee,
Seung-Jin Lee,
Sam-Pin Lee,
Naila Boby,
Kyoungho Suk,
Biruk Tesfaye Birhanu,
Seung-Chun Park
Affiliations
Md. Sekendar Ali
Department of Biomedical Science and Department of Pharmacology, School of Medicine, Brain Science and Engineering Institute, Kyungpook National University, Daegu 41944, Korea
Eon-Bee Lee
Laboratory of Veterinary Pharmacokinetics and Pharmacodynamics, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea
Seung-Jin Lee
Development and Reproductive Toxicology Research Group, Korea Institute of Toxicology, Daejeon 34114, Korea
Sam-Pin Lee
Department of Food Science and Technology, Keimyung University, Daegu 42601, Korea
Naila Boby
Laboratory of Veterinary Pharmacokinetics and Pharmacodynamics, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea
Kyoungho Suk
Department of Biomedical Science and Department of Pharmacology, School of Medicine, Brain Science and Engineering Institute, Kyungpook National University, Daegu 41944, Korea
Biruk Tesfaye Birhanu
Laboratory of Veterinary Pharmacokinetics and Pharmacodynamics, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea
Seung-Chun Park
Laboratory of Veterinary Pharmacokinetics and Pharmacodynamics, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea
The present study aimed to assess the immunomodulatory effects of fermented Aronia melanocarpa extract (FAME) on RAW 264.7 cells and BALB/c mice. Aronia melanocarpa fruit was fermented with Lactobacillus plantarum EJ2014 by adding yeast extract and monosodium glutamate for 9 days at 30 °C to produce γ-aminobutyric acid (GABA). After fermentation, significant GABA production was noted, along with minerals, polyphenols, and flavonoids (p p + and CD8+ T-cell expression, and lymphocyte proliferation. Furthermore, it increased IFN-γ, IL-2, and IL-4 cytokine levels in a dose-dependent manner (p p < 0.05). These results indicate that FAME fortified with GABA including bioactive compounds exerts anti-inflammatory effects by inhibiting proinflammatory cytokines in RAW 264.7 cells and modulates immune response in mice. Thus, FAME could be a potential therapeutic agent for inflammatory disorders.
