miR-214 inhibits the osteogenic differentiation of dental follicle cells in vitro

WANG Zhiheng; ZUO Jie; WANG Mengqi; ZHU Shaojun; LIU Yishan

doi:10.12016/j.issn.2096-1456.2020.03.002

口腔疾病防治 (Mar 2020)

miR-214 inhibits the osteogenic differentiation of dental follicle cells in vitro

WANG Zhiheng,
ZUO Jie,
WANG Mengqi,
ZHU Shaojun,
LIU Yishan

Affiliations

WANG Zhiheng: Department of Pediatric Dentistry and Oral Protection, the First Affiliated Hospital/Affiliated Dental Hospital of Xinjiang Medical University, Xinjiang Uyghur Autonomous Region Institute of Stomatology, Urumqi 830054, China
ZUO Jie: Department of Pediatric Dentistry and Oral Protection, the First Affiliated Hospital/Affiliated Dental Hospital of Xinjiang Medical University, Xinjiang Uyghur Autonomous Region Institute of Stomatology, Urumqi 830054, China
WANG Mengqi: Department of Pediatric Dentistry and Oral Protection, the First Affiliated Hospital/Affiliated Dental Hospital of Xinjiang Medical University, Xinjiang Uyghur Autonomous Region Institute of Stomatology, Urumqi 830054, China
ZHU Shaojun: Department of Pediatric Dentistry and Oral Protection, the First Affiliated Hospital/Affiliated Dental Hospital of Xinjiang Medical University, Xinjiang Uyghur Autonomous Region Institute of Stomatology, Urumqi 830054, China
LIU Yishan: Department of Pediatric Dentistry and Oral Protection, the First Affiliated Hospital/Affiliated Dental Hospital of Xinjiang Medical University, Xinjiang Uyghur Autonomous Region Institute of Stomatology, Urumqi 830054, China

DOI: https://doi.org/10.12016/j.issn.2096-1456.2020.03.002
Journal volume & issue: Vol. 28, no. 3
pp. 146 – 152

Abstract

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Objective To investigate the effect of miR-214 on the osteogenic differentiation of dental follicle cells (DFCs). Methods Purified DFCs were cultured in vitro by bidirectional differential passage, with the untransfected DFCs as the control group (DFCs group). The expression of miR-214-3p in DFCs was upregulated and downregulated by transfection of miR-214-3p(miR-214 mimics group) or miR-214-3p inhibitors(miR-214 inhibitor group) into DFCs. The expression levels of miR-214, alkaline phosphatase (ALP), osteonectin (OSN) and runt-related transcription factor-2(RUNX-2) were detected by qRT-PCR after 7 days of osteogenesis induction, the protein expression levels of RUNX-2 and β-catenin were detected by western blot, and the formation of mineralized nodules was observed with alizarin red staining after 14 days of osteogenesis induction. Results Compared with the DFCs group, in the miR-214 mimics group, the expression of miR-214 was upregulated after 7 days of osteogenesis induction. The mRNA expression of ALP, OSN and RUNX-2 in the miR-214 mimics group was lower than that in the DFCs group, but only ALP in the two groups was statistically significant (P > 0.05); the mRNA expression of ALP, OSN and RUNX-2 in the miR-214 inhibitor group was higher than that in the DFCs group, and the difference was statistically significant (P < 0.05). The protein expression of RUNX-2 and β-catenin in the miR-214 mimics group was lower than that in the miR-214 inhibitor group. The number of calcified nodules in the miR-214 mimics group was significantly less than that in the DFCs group, while that in the miR-214 inhibitor group was significantly higher than that in the DFCs group. Conclusion The upregulation of miR-214 can downregulate the expression of β-catenin, can inhibit the expression of ALP, OSN and RUNX-2 related to osteogenesis, and can inhibit osteogenic differentiation. The downregulation of miR-214 demonstrated the opposite results; miR-214 may downregulate the expression of β-catenin and inhibit the osteogenic differentiation of DFCs.

Published in 口腔疾病防治

ISSN: 2096-1456 (Print)
Publisher: Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases
Country of publisher: China
LCC subjects: Medicine
Website: http://www.kqjbfz.com/EN/2096-1456/home.shtml

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