Generation of 3 human induced pluripotent stem cell lines LUMCi005-A, B and C from a Hereditary Cerebral Hemorrhage with Amyloidosis-Dutch type patient

Elena Daoutsali; Ronald A.M. Buijsen; Simone van de Pas; Anke 't Jong; Harald Mikkers; Tom Brands; Bert Eussen; Annelies de Klein; Linda M. van der Graaf; Barry A. Pepers; Christian Freund; Gisela M. Terwindt; Valeria V. Orlova; Willeke M.C. van Roon-Mom

Stem Cell Research (Jan 2019)

Generation of 3 human induced pluripotent stem cell lines LUMCi005-A, B and C from a Hereditary Cerebral Hemorrhage with Amyloidosis-Dutch type patient

Elena Daoutsali,
Ronald A.M. Buijsen,
Simone van de Pas,
Anke 't Jong,
Harald Mikkers,
Tom Brands,
Bert Eussen,
Annelies de Klein,
Linda M. van der Graaf,
Barry A. Pepers,
Christian Freund,
Gisela M. Terwindt,
Valeria V. Orlova,
Willeke M.C. van Roon-Mom

Affiliations

Elena Daoutsali: Department of Human Genetics, LUMC, Leiden, The Netherlands; Corresponding author.
Ronald A.M. Buijsen: Department of Human Genetics, LUMC, Leiden, The Netherlands
Simone van de Pas: LUMC hiPSC Core Facility, Department of Anatomy and Embryology, LUMC, Leiden, The Netherlands
Anke 't Jong: LUMC hiPSC Core Facility, Department of Anatomy and Embryology, LUMC, Leiden, The Netherlands
Harald Mikkers: LUMC hiPSC Core Facility, Department of Anatomy and Embryology, LUMC, Leiden, The Netherlands
Tom Brands: Department of Clinical Genetics, Erasmus MC, Rotterdam, The Netherlands; Department of Ophthalmology, Erasmus MC, Rotterdam, The Netherlands
Bert Eussen: Department of Clinical Genetics, Erasmus MC, Rotterdam, The Netherlands
Annelies de Klein: Department of Clinical Genetics, Erasmus MC, Rotterdam, The Netherlands
Linda M. van der Graaf: Department of Human Genetics, LUMC, Leiden, The Netherlands
Barry A. Pepers: Department of Human Genetics, LUMC, Leiden, The Netherlands
Christian Freund: LUMC hiPSC Core Facility, Department of Anatomy and Embryology, LUMC, Leiden, The Netherlands
Gisela M. Terwindt: Department of Neurology, LUMC, Leiden, The Netherlands
Valeria V. Orlova: Department of Anatomy and Embryology, LUMC, Leiden, The Netherlands
Willeke M.C. van Roon-Mom: Department of Human Genetics, LUMC, Leiden, The Netherlands

Journal volume & issue: Vol. 34

Abstract

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Hereditary Cerebral Hemorrhage with Amyloidosis-Dutch type (HCHWA-D) is an autosomal dominant hereditary disease caused by a point mutation in exon 17 of the APP gene. We generated human induced pluripotent stem cells (hiPSCs) from a symptomatic HCHWA-D patient by using non-integrating Sendai virus (SeV). The newly generated hiPSCs express all pluripotency markers, have a normal karyotype, carry the Dutch mutation, can differentiate in the three germ layers in vitro and are SeV free.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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