Scientific Reports (Jun 2022)

Mitochondrial complex I dysfunction alters the balance of soluble and membrane-bound TNF during chronic experimental colitis

  • Ainize Peña-Cearra,
  • Miguel Angel Pascual-Itoiz,
  • Jose Luis Lavín,
  • Miguel Fuertes,
  • Itziar Martín-Ruiz,
  • Janire Castelo,
  • Ainhoa Palacios,
  • Diego Barriales,
  • Asier Fullaondo,
  • Ana M Aransay,
  • Hector Rodríguez,
  • Juan Anguita,
  • Leticia Abecia

DOI
https://doi.org/10.1038/s41598-022-13480-y
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 10

Abstract

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Abstract Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors. MCJ is a mitochondrial protein that regulates the metabolic status of macrophages and their response to translocated bacteria. Previously, an acute murine model of DSS-induced colitis showed increased disease severity due to MCJ deficiency. Unexpectedly, we now show that MCJ-deficient mice have augmented tumor necrosis factor α converting enzyme (TACE) activity in the context of chronic inflammation. This adaptative change likely affects the balance between soluble and transmembrane TNF and supports the association of the soluble form and a milder phenotype. Interestingly, the general shifts in microbial composition previously observed during acute inflammation were absent in the chronic model of inflammation in MCJ-deficient mice. However, the lack of the mitochondrial protein resulted in increased alpha diversity and the reduction in critical microbial members associated with inflammation, such as Ruminococcus gnavus, which could be associated with TACE activity. These results provide evidence of the dynamic metabolic adaptation of the colon tissue to chronic inflammatory changes mediated by the control of mitochondrial function.