Design strategy for serine hydroxymethyltransferase probes based on retro-aldol-type reaction

Hiroshi Nonaka; Yuki Nakanishi; Satoshi Kuno; Tomoki Ota; Kentaro Mochidome; Yutaro Saito; Fuminori Sugihara; Yoichi Takakusagi; Ichio Aoki; Satoru Nagatoishi; Kouhei Tsumoto; Shinsuke Sando

doi:10.1038/s41467-019-08833-7

Nature Communications (Feb 2019)

Design strategy for serine hydroxymethyltransferase probes based on retro-aldol-type reaction

Hiroshi Nonaka,
Yuki Nakanishi,
Satoshi Kuno,
Tomoki Ota,
Kentaro Mochidome,
Yutaro Saito,
Fuminori Sugihara,
Yoichi Takakusagi,
Ichio Aoki,
Satoru Nagatoishi,
Kouhei Tsumoto,
Shinsuke Sando

Affiliations

Hiroshi Nonaka: Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
Yuki Nakanishi: Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
Satoshi Kuno: Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
Tomoki Ota: Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
Kentaro Mochidome: Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
Yutaro Saito: Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
Fuminori Sugihara: Core Instrumentation Facility, Immunology Frontier Research Center and Research Institute for Microbial Diseases, Osaka University
Yoichi Takakusagi: National Institute of Radiological Sciences (NIRS), National Institutes for Quantum and Radiological Science and Technology (QST)
Ichio Aoki: National Institute of Radiological Sciences (NIRS), National Institutes for Quantum and Radiological Science and Technology (QST)
Satoru Nagatoishi: Medical Proteomics Laboratory, Institute of Medical Science, The University of Tokyo, 4-6-1, Shiroganedai, Minato-ku
Kouhei Tsumoto: Medical Proteomics Laboratory, Institute of Medical Science, The University of Tokyo, 4-6-1, Shiroganedai, Minato-ku
Shinsuke Sando: Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo

DOI: https://doi.org/10.1038/s41467-019-08833-7
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 10

Abstract

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The enzyme serine hydroxymethyltransferase (SHMT) has been implicated in several diseases, however is hard to investigate. Here, the authors used a design strategy based on the retro-aldol-type reaction catalyzed by SHMT to develop SHMT-responsive fluorescence and 19F NMR molecular probes.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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