Immune Response Modulation by HPV16 Oncoproteins in Lung Cancer: Insights from Clinical and In Vitro Investigations
Bianca de França São Marcos,
Daffany Luana dos Santos,
Georon Ferreira de Sousa,
Leonardo Carvalho de Oliveira Cruz,
Bárbara Rafaela da Silva Barros,
Matheus Gardini Amâncio Marques de Sena,
Vanessa Emanuelle Pereira Santos,
Talita Helena de Araújo Oliveira,
Cristiane Moutinho Lagos de Melo,
Antonio Carlos de Freitas
Affiliations
Bianca de França São Marcos
Laboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Cidade Universitária Recife, Recife 50670-901, PE, Brazil
Daffany Luana dos Santos
Laboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Cidade Universitária Recife, Recife 50670-901, PE, Brazil
Georon Ferreira de Sousa
Keizo Asami Immunopathology Laboratory, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Cidade Universitária Recife, Recife 50670-901, PE, Brazil
Leonardo Carvalho de Oliveira Cruz
Keizo Asami Immunopathology Laboratory, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Cidade Universitária Recife, Recife 50670-901, PE, Brazil
Bárbara Rafaela da Silva Barros
Keizo Asami Immunopathology Laboratory, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Cidade Universitária Recife, Recife 50670-901, PE, Brazil
Matheus Gardini Amâncio Marques de Sena
Laboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Cidade Universitária Recife, Recife 50670-901, PE, Brazil
Vanessa Emanuelle Pereira Santos
Laboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Cidade Universitária Recife, Recife 50670-901, PE, Brazil
Talita Helena de Araújo Oliveira
Patrick G Jonhston Centre for Cancer Research, Queen’s University Belfast, Belfast BT7 1NN, UK
Cristiane Moutinho Lagos de Melo
Keizo Asami Immunopathology Laboratory, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Cidade Universitária Recife, Recife 50670-901, PE, Brazil
Antonio Carlos de Freitas
Laboratory of Molecular Studies and Experimental Therapy, Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, Cidade Universitária Recife, Recife 50670-901, PE, Brazil
Lung cancer has the highest mortality rates worldwide, and Human Papillomavirus (HPV) has been associated with its carcinogenesis. In this study, HPV16 genes’ expressions were investigated in patient samples, along with the immunological response promoted by lymphocytes and monocytes in A549 cells transfected with HPV oncogenes and co-cultured with PBMC. An increase in the expression of E5 was observed in the patients’ samples. In the in vitro analysis, a decrease in the number of monocytes and cytotoxic cells was observed when co-stimulated by E6 and E7, and it promoted an increase in the Th2 profile. In contrast, the high proliferation of cytotoxic cells in A549 cells transfected with E5, associated with the high expression of costimulatory molecules in monocytes, suggests a low capacity of E5 to inhibit the presentation of antigens by antigen-presenting cells (APC) and a possible use of E5 in future therapeutic strategies against lung cancers associated with HPV.
