Известия высших учебных заведений. Поволжский регион: Естественные науки (Oct 2022)
The effect of ethanol consumption on the carboxypeptidase E activity on different age rats posterity
Abstract
Background. The consumption of ethanol by dams strongly effects the offspring’s body, its health both at birth and throughout life. This effect has a particularly strong effect on the functioning of various regulatory systems, in particular hypothalamicpituitary- adrenal and opioidergic. Carboxypeptidase E (CPE, EC 3.4.17.10, NF–α1) participates in the final stage of the formation of effectors of these systems – biologically active peptides. Therefore, the purpose of the work is to study the effect of ethanol consumption by mothers on the activity of this enzyme. Materials and methods. Experiments were carried out on males and females of white mongrel rats (Rattusnorvegicus) of different ages. 2 groups of rats were used in the study: prenatally alcoholized (PA, experimental) and control. The activity of CPE was determined by the fluorometric method of Fricker et al. with dancyl-Phe-Ala-Arg substrate for inhibition by guanidinoethylmercaptosuccinic acid. Protein was determined by the Lowry method. Results. PA significantly influenced the activity of CPE in the hypothalamus, striatum and adrenal glands, age – in all the studied departments, the interaction of PA and sex – in the hypothalamus, the interaction of PA and age – in the hypothalamus and striatum, the interaction of sex and age – in all the studied departments, the interaction of all three factors – in the striatum and adrenal glands. Conclusions. Thus, the consumption of ethanol by dams had a significant effect on the activity of CPE in the hypothalamus, striatum and adrenal glands of the offspring, and in the hypothalamus and striatum this effect depended on the sex and age of the animals. Probably, changes in enzyme activity contribute to neurophysiological, neurochemical, neurohumoral and behavioral disorders caused by PA.
Keywords
