Морфологія (Jan 2011)
Histogenesis of epicardium and coronary vessels endothelium of chick embryo on early stages of prenatal ontogenesis at normal condition s and during liver bud ablation
Abstract
There is a close interrelationship between embryonic development of epicardium, coronary endothe lium and liver, but the nature of such interrelations is still unknown. To reveal the role of liver in epicardium and coronary vessels formation we stopped its development by injection of aminoguanidine sulfate into the yolk sack of chick embryos on 14 stage of deve lopment by HH (first group) - to prevent contact between proepicardium and liver, and on 16 stage (seco nd group) to allow contact between proepicardium and liver during a short period of time. As a result in first experimental group we observed complete absence of epicardium, deficit of volume of myocardium and endocardial structures. In second experimental group formation of epicardium has begun, but subepicardial space was devoid of mesenchymal cells; deficit of volume of myocardium and endocardial structures also was registered. Another difference was formation of coronary vessels, but their total volume was much smaller then under normal conditions. All embryos died by the end of the third (first group) and fifth (second group) day of incubation.
