Nature Communications (Jun 2024)
descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques
- Kohei Otomo,
- Takaki Omura,
- Yuki Nozawa,
- Steven J. Edwards,
- Yukihiko Sato,
- Yuri Saito,
- Shigehiro Yagishita,
- Hitoshi Uchida,
- Yuki Watakabe,
- Kiyotada Naitou,
- Rin Yanai,
- Naruhiko Sahara,
- Satoshi Takagi,
- Ryohei Katayama,
- Yusuke Iwata,
- Toshiro Shiokawa,
- Yoku Hayakawa,
- Kensuke Otsuka,
- Haruko Watanabe-Takano,
- Yuka Haneda,
- Shigetomo Fukuhara,
- Miku Fujiwara,
- Takenobu Nii,
- Chikara Meno,
- Naoki Takeshita,
- Kenta Yashiro,
- Juan Marcelo Rosales Rocabado,
- Masaru Kaku,
- Tatsuya Yamada,
- Yumiko Oishi,
- Hiroyuki Koike,
- Yinglan Cheng,
- Keisuke Sekine,
- Jun-ichiro Koga,
- Kaori Sugiyama,
- Kenichi Kimura,
- Fuyuki Karube,
- Hyeree Kim,
- Ichiro Manabe,
- Tomomi Nemoto,
- Kazuki Tainaka,
- Akinobu Hamada,
- Hjalmar Brismar,
- Etsuo A. Susaki
Affiliations
- Kohei Otomo
- Department of Biochemistry and Systems Biomedicine, Juntendo University Graduate School of Medicine
- Takaki Omura
- Department of Biochemistry and Systems Biomedicine, Juntendo University Graduate School of Medicine
- Yuki Nozawa
- Biochemistry II, Juntendo University School of Medicine
- Steven J. Edwards
- Science for Life Laboratory, Department of Applied Physics, KTH Royal Institute of Technology
- Yukihiko Sato
- Department of Biochemistry and Systems Biomedicine, Juntendo University Graduate School of Medicine
- Yuri Saito
- Department of Biochemistry and Systems Biomedicine, Juntendo University Graduate School of Medicine
- Shigehiro Yagishita
- Department of Pharmacology and Therapeutics, Fundamental Innovative Oncology Core, National Cancer Center Research Institute
- Hitoshi Uchida
- Department of System Pathology for Neurological Disorders, Brain Research Institute, Niigata University
- Yuki Watakabe
- Division of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural Sciences
- Kiyotada Naitou
- Department of Basic Veterinary Science, Joint Faculty of Veterinary Medicine, Kagoshima University
- Rin Yanai
- Advanced Neuroimaging Center, Institute for Quantum Medical Sciences, National Institutes for Quantum Science and Technology
- Naruhiko Sahara
- Advanced Neuroimaging Center, Institute for Quantum Medical Sciences, National Institutes for Quantum Science and Technology
- Satoshi Takagi
- Division of Experimental Chemotherapy, Cancer Chemotherapy Center, Japanese Foundation for Cancer Research
- Ryohei Katayama
- Division of Experimental Chemotherapy, Cancer Chemotherapy Center, Japanese Foundation for Cancer Research
- Yusuke Iwata
- Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo
- Toshiro Shiokawa
- Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo
- Yoku Hayakawa
- Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo
- Kensuke Otsuka
- Biology and Environmental Chemistry Division, Sustainable System Research Laboratory, Central Research Institute of Electric Power Industry
- Haruko Watanabe-Takano
- Department of Molecular Pathophysiology, Institute for Advanced Medical Sciences, Nippon Medical School
- Yuka Haneda
- Department of Molecular Pathophysiology, Institute for Advanced Medical Sciences, Nippon Medical School
- Shigetomo Fukuhara
- Department of Molecular Pathophysiology, Institute for Advanced Medical Sciences, Nippon Medical School
- Miku Fujiwara
- Department of Developmental Biology, Graduate School of Medical Sciences, Kyushu University
- Takenobu Nii
- Department of Developmental Biology, Graduate School of Medical Sciences, Kyushu University
- Chikara Meno
- Department of Developmental Biology, Graduate School of Medical Sciences, Kyushu University
- Naoki Takeshita
- Anatomy and Developmental Biology, Kyoto Prefectural University of Medicine
- Kenta Yashiro
- Anatomy and Developmental Biology, Kyoto Prefectural University of Medicine
- Juan Marcelo Rosales Rocabado
- Division of Bio-prosthodontics, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University
- Masaru Kaku
- Division of Bio-prosthodontics, Faculty of Dentistry & Graduate School of Medical and Dental Sciences, Niigata University
- Tatsuya Yamada
- Department of Biochemistry, University of Nebraska-Lincoln
- Yumiko Oishi
- Department of Meidical Biochemistry, Tokyo Medical and Dental University
- Hiroyuki Koike
- Department of Meidical Biochemistry, Tokyo Medical and Dental University
- Yinglan Cheng
- Department of Meidical Biochemistry, Tokyo Medical and Dental University
- Keisuke Sekine
- Laboratory of Cancer Cell Systems, National Cancer Center Research Institute
- Jun-ichiro Koga
- The Second Department of Internal Medicine, University of Occupational and Environmental Health
- Kaori Sugiyama
- Institute for Advanced Research of Biosystem Dynamics, Research Institute for Science and Engineering, Waseda University
- Kenichi Kimura
- Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba
- Fuyuki Karube
- Lab of Histology and Cytology, Graduate School of Medicine, Hokkaido University
- Hyeree Kim
- Department of Systems Medicine, Graduate School of Medicine, Chiba University
- Ichiro Manabe
- Department of Systems Medicine, Graduate School of Medicine, Chiba University
- Tomomi Nemoto
- Division of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural Sciences
- Kazuki Tainaka
- Department of System Pathology for Neurological Disorders, Brain Research Institute, Niigata University
- Akinobu Hamada
- Department of Pharmacology and Therapeutics, Fundamental Innovative Oncology Core, National Cancer Center Research Institute
- Hjalmar Brismar
- Science for Life Laboratory, Department of Applied Physics, KTH Royal Institute of Technology
- Etsuo A. Susaki
- Department of Biochemistry and Systems Biomedicine, Juntendo University Graduate School of Medicine
- DOI
- https://doi.org/10.1038/s41467-024-49131-1
- Journal volume & issue
-
Vol. 15,
no. 1
pp. 1 – 19
Abstract
Abstract Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM for cleared specimens), a low-cost ($20,000–50,000), low-expertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a three-dimensional manner. Academically open-sourced ( https://github.com/dbsb-juntendo/descSPIM ), descSPIM allows routine three-dimensional imaging of cleared samples in minutes. Thus, the dissemination of descSPIM will accelerate biomedical discoveries driven by tissue clearing technologies.
