Optimization of HS-SPME-GC/MS Analysis of Volatile Components in Camellia Seed Oil

Abstract

An analysis method for the volatile components of squeezed Camellia oleifera seed oil was established by using automatic sample preparation-headspace solid phase microextraction (HS-SPME)combined with gas chromatography-mass spectrometry (GC/MS) technology.The optimum extraction conditions are:Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS) solid-phase microextraction column,extraction temperature 60 ℃,extraction time 50 min,resolution time 5 min,compared with traditional manual water bath extraction method,it has higher total peak area and main peak area; A total of 47 volatile components in pressed camellia seed oil were identified and quantified using this method,of which aldehydes are the main volatile components in camellia seed oil; The characteristic flavor compounds of pressed camellia seed oil obtained by relative odor activity value (ROAV) analysis are 2-hydro-3-hydroxy-4,4-dimethyl-2(3H)furanone,dihydroxy-2(3H)furanone,2-methyl Propionaldehyde,phenylacetaldehyde,nonanal,n-hexanal,trans-2-nonenal,decanal,1-heptanol,3-ethyl-3,5-dimethyl-pyrazine.

Keywords

• camellia seed oil
• solid phase microextraction
• volatile components
• relative odor activity value