Antioxidant Efficacy and “In Vivo” Safety of a Bentonite/Vitamin C Hybrid
Dayaris Hernández,
Anaela Montalvo,
Irela Pérez,
Clarence Charnay,
Rita Sánchez-Espejo,
Pilar Cerezo,
César Viseras,
Serena Riela,
Giuseppe Cinà,
Aramis Rivera
Affiliations
Dayaris Hernández
Zeolites Engineering Laboratory, Institute of Materials Science and Technology (IMRE), University of Havana, Havana 10400, Cuba
Anaela Montalvo
Department of Drugs Technology and Control, Institute of Pharmacy and Food (IFAL), University of Havana, Havana 13600, Cuba
Irela Pérez
Department of Drugs Technology and Control, Institute of Pharmacy and Food (IFAL), University of Havana, Havana 13600, Cuba
Clarence Charnay
Institut Charles Gerhardt de Montpellier, CNRS UMR 5253, Université de Montpellier, 34095 Montpellier, France
Rita Sánchez-Espejo
Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, Campus of Cartuja, 18071 Granada, Spain
Pilar Cerezo
Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, Campus of Cartuja, 18071 Granada, Spain
César Viseras
Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, Campus of Cartuja, 18071 Granada, Spain
Serena Riela
Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Viale delle Scienze, Ed. 17, 90128 Palermo, Italy
Giuseppe Cinà
Department of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Viale delle Scienze, Ed. 17, 90128 Palermo, Italy
Aramis Rivera
Zeolites Engineering Laboratory, Institute of Materials Science and Technology (IMRE), University of Havana, Havana 10400, Cuba
L-ascorbic acid (LAA), commonly known as vitamin C, is an excellent and recognized antioxidant molecule used in pharmaceutical and cosmetic formulations. Several strategies have been developed in order to preserve its chemical stability, connected with its antioxidant power, but there is little research regarding the employment of natural clays as LAA host. A safe bentonite (Bent)—which was verified by in vivo ophthalmic irritability and acute dermal toxicity assays—was used as carrier of LAA. The supramolecular complex between LAA and clay may constitute an excellent alternative, since the molecule integrity does not seem to be affected, at least from the point of view of its antioxidant capacity. The Bent/LAA hybrid was prepared and characterized through ultraviolet (UV) spectroscopy, X-ray diffraction (XRD), infrared (IR) spectroscopy, thermogravimetric analysis (TG/DTG) and zeta potential measurements. Photostability and antioxidant capacity tests were also performed. The LAA incorporation into Bent clay was demonstrated, as well as the drug stability due to the Bent photoprotective effect onto the LAA molecule. Moreover, the antioxidant capacity of the drug in the Bent/LAA composite was confirmed.
