Proceedings (Nov 2017)

The Effect of Tocopherol-α on the Cell Viability in Caco-2 Cell Line

  • Ayşenur Gök,
  • Efe Kurtdede,
  • Remzi Soner Cengiz,
  • Görkem Kısmalı,
  • Tevhide Sel

DOI
https://doi.org/10.3390/proceedings1101062
Journal volume & issue
Vol. 1, no. 10
p. 1062

Abstract

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Tocopherols, the major forms of vitamin E, are a family of fat-soluble phenolic compounds. Each tocopherol contains a chromanol ring system and a phytyl chain containing 16 carbons. Tocopherols, as effective antioxidants, have been proposed to protect against carcinogenesis. Colon carsinoma cell line were treated with tocopherol-α (0, 3.12, 6.25, 12.5, 25, 50, 100, 200 uM). Cell viability and migration were examined. Cell viability was determined MTT assay and cell migration was determined by wound healing assay. Caco-2 cells were treated with for 24 h, 48 h and 72 h incubation. There is a significant decrease was observed at 50, 100 and 200 µM for 48 h and 72 h incubation on cell viability in the MTT assay. Wound healing method observed decrease on migration at 12, 5, 25 and 50 µM in 24 h. These results suggest that tocopherol-α have promising antiproliferative effect on cell viability for research on cancer.

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