An Extraction Process Based on the Collaborative Extraction of <i>Coptis chinensis</i> Franch. Phytoconstituents Using a Deep Eutectic Solvent and an Organic Solvent
Cheng Liu,
Fangyuan Gong,
Zhengwei Xiong,
Cun Wang,
Xinhe Ran,
Jiahua Ran,
Runzi Li,
Yangjin Ou,
Qingqing Xia,
Pei Wei,
Jin Guo
Affiliations
Cheng Liu
Chongqing Field Scientific Observation and Research Station for Authentic Traditional Chinese Medicine in the Tree Gorges Reservoir Area, Chongqing University of Education, Chongqing 400067, China
Fangyuan Gong
College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
Zhengwei Xiong
College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
Cun Wang
College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
Xinhe Ran
College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
Jiahua Ran
College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
Runzi Li
College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
Yangjin Ou
College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
Qingqing Xia
Chongqing Field Scientific Observation and Research Station for Authentic Traditional Chinese Medicine in the Tree Gorges Reservoir Area, Chongqing University of Education, Chongqing 400067, China
Pei Wei
Chongqing Field Scientific Observation and Research Station for Authentic Traditional Chinese Medicine in the Tree Gorges Reservoir Area, Chongqing University of Education, Chongqing 400067, China
Jin Guo
Agricultural Development Service Center of Kaizhou District, Chongqing 400067, China
A low-cost method for the simultaneous extraction of alkaloids and water-insoluble flavonoids and esters from Coptis chinensis Franch. (Abbrev. C. chinensis) was explored to provide a reference for the production of green plant-based preparations and traditional Chinese medicine formula granules. A combined extraction method with the deep eutectic solvents (DESs) of choline chloride and urea (molar mass ratio of 1:2) and organic solvent ethanol was used, supplemented by ultrasonic-assisted extraction (ultrasonic power: 150 W; ultrasonic temperature: 60 °C; treatment time: 15 min). The extraction efficiency of the 50% DES (choline chloride and urea) aqueous solution for berberine, palmatine, jatrorrhizine, and magnoflorine was found to be the highest and was superior to traditional ultrasonic extraction and water bath reflux extraction methods. Furthermore, the flavonoids and esters from C. chinensis residue were extracted using ethanol. The results from high-performance liquid chromatography and gas chromatography–mass spectrometry indicated a high extraction efficiency overall.
