Therapeutic effect of hydroalcoholic extract of Moringa oleifera and sulfurphane on melanoma caused by B16f10 cell line in mouse model C57bl/6

Abstract

Background: Moringa oleifera, an herbal medicine has some antitumor effects on various cancers.Objectives: This study aimed to investigate the effect of plants extract on anti-inflammatory, antioxidant properties on metastasis.Methods: At first, the phenolic compounds in Moringa oleifera were confirmed by HPLC and spectrophotometer methods, then the anticancer effect of phenolic compounds sulforaphane and Moringa oleifera on B16F10 cancer cells was investigated. Then, induction of melanoma tumor by B16F10 cell line was induced in C57BL6 mice, and the mice were treated with extract. Quercetin, gallic acid, and caffeic acid were identified in extract.Results: The rate of cell death in the sulforaphane-exposed cell group was lower than in the cell treated with Moringa oleifera group. Tumor volume in all three doses of 0.04, 0.08, 0.02 g Moringa extract or phenolic compounds decreased significantly after one week and two weeks (P<0.001). Tumor volume decreased by 0.02 g more than the other two doses (P <0.001).Conclusion: This study shows the positive effect of antioxidants in Moringa oleifera extract in reducing the life of cancer cells and tumor volume in mice. With further studies, Moringa oleifera can be introduced as an anticancer drug with more confidence. It is recommended to perform studies in human tests.

Keywords

• moringa oleifera
• sulforaphane, melanoma, b16f10 melanoma cell line