Phytochemical Composition and Antimicrobial and Antibiofilm Effect of Myrciaria cauliflora Hydroethanolic Extract against Staphylococcus aureus and Acinetobacter baumannii

Luciane Dias de Oliveira; Ana Luisa Monteiro Ribeiro; Sthéfani de Oliveira Dias; Geovani Moreira da Cruz; Raquel Teles de Menezes; Lara Steffany de Carvalho; Mariana Gadelho Gimenez Diamantino; Thaís Cristine Pereira; Maria Cristina Marcucci; Amjad Abu Hasna

doi:10.3390/mps7040060

Methods and Protocols (Aug 2024)

Phytochemical Composition and Antimicrobial and Antibiofilm Effect of Myrciaria cauliflora Hydroethanolic Extract against Staphylococcus aureus and Acinetobacter baumannii

Luciane Dias de Oliveira,
Ana Luisa Monteiro Ribeiro,
Sthéfani de Oliveira Dias,
Geovani Moreira da Cruz,
Raquel Teles de Menezes,
Lara Steffany de Carvalho,
Mariana Gadelho Gimenez Diamantino,
Thaís Cristine Pereira,
Maria Cristina Marcucci,
Amjad Abu Hasna

Affiliations

Luciane Dias de Oliveira: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Campus of São José dos Campos, São Paulo 12245-000, Brazil
Ana Luisa Monteiro Ribeiro: Campus São José dos Campos, Universidade Paulista—UNIP, Highway Presidente Dutra, km 157.5, South Lane, São José dos Campos, São Paulo 12240-420, Brazil
Sthéfani de Oliveira Dias: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Campus of São José dos Campos, São Paulo 12245-000, Brazil
Geovani Moreira da Cruz: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Campus of São José dos Campos, São Paulo 12245-000, Brazil
Raquel Teles de Menezes: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Campus of São José dos Campos, São Paulo 12245-000, Brazil
Lara Steffany de Carvalho: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Campus of São José dos Campos, São Paulo 12245-000, Brazil
Mariana Gadelho Gimenez Diamantino: Department of Restorative Dentistry, Endodontics Division, Institute of Science and Technology, São Paulo State University, Campus of São José dos Campos, São Paulo 12245-000, Brazil
Thaís Cristine Pereira: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Campus of São José dos Campos, São Paulo 12245-000, Brazil
Maria Cristina Marcucci: Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Campus of São José dos Campos, São Paulo 12245-000, Brazil
Amjad Abu Hasna: Department of Restorative Dentistry, Endodontics Division, Institute of Science and Technology, São Paulo State University, Campus of São José dos Campos, São Paulo 12245-000, Brazil

DOI: https://doi.org/10.3390/mps7040060
Journal volume & issue: Vol. 7, no. 4
p. 60

Abstract

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Staphylococcus aureus and Acinetobacter baumannii are opportunistic pathogens, and both are involved in different oral infections. This work aimed to analyze the phytochemical composition of Myrciaria cauliflora hydroethanolic extract and to evaluate its antimicrobial and antibiofilm action against Staphylococcus aureus (ATCC 6538) and Acinetobacter baumannii (ATCC 19606; multi-resistant clinical strains 58004, 50098, 566006, and H557). Myrciaria cauliflora hydroethanolic extract was prepared, and the content of soluble solids, flavonoids, and phenols was quantified. High-performance liquid chromatography (HPLC) was performed later. The minimum inhibitory concentration was determined using the broth microdilution method according to the Clinical and Laboratory Standards Institute, standard M7-A6, and subsequently, its minimum bactericidal concentration was determined. Then, the most effective concentrations were analyzed against biofilms. Statistical analysis was performed using the ANOVA method with Tukey’s test. The soluble solids content in the prepared hydroethanolic extract of M. cauliflora was 2.22%. Additionally, the total flavonoid content, measured using the quercetin standard curve, was 0.040 mg/mL. Furthermore, the total phenol content, determined using the gallic acid standard curve, was 0.729 mg/mL. HPLC analysis presented peaks of gallic acid (11.80 m), p-coumaric acid (12.09 m), cinnamic acid derivative (19.02 m), and ellagic acid (29.83 m). The extract demonstrated antimicrobial and antibiofilm action against all tested strains. However, the most effective antibacterial concentration against all the tested bacteria was 5.55 mg/mL. Therefore, these chemical components justify that M. cauliflora hydroethanolic extract is effective in reducing biofilm formation in S. aureus (standard strain) and A. baumannii (standard and clinical strains).

Published in Methods and Protocols

ISSN: 2409-9279 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: https://www.mdpi.com/journal/mps

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