The influence of cranioskeletal injury on the dynamics of the enzyme link of antioxidant protection in the renal cortex and medulla under bilateral removal of the gonades during late manifestations of traumatic disease

Abstract

In recent years, there has been a significant increase in the share of women in the postmenopausal period. Natural involution, which is based on lower estrogen levels, significantly changes the reactivity and resistance of a woman's body to various environmental factors. However, until recently, researchers have ignored the study of the role of estrogen deficiency in the violation of antioxidant protection in the conditions of traumatic illness, which would contribute to the search for directions of pathogenetically justified correction. The aim of the study – to determine the effect of bilateral ovariectomy on the enzymatic link of antioxidant protection in the renal cortex and medulla in the late period of cranioskeletal injury (CSI). Materials and Methods. The experiments were performed on 54 nonlinear white female rats weighing 200–220 g. The hypoestrogenic state was performed by surgical removal of the gonads with subsequent simulation of CSI. After 1 and 2 months of the post-traumatic period, superoxide dismutase (SOD) and catalase activity were determined in the cortex and cerebral layers of the kidney. Results. After 1 month of the post-traumatic period in animals without gonadectomy SOD and catalase activity of the renal cortex and medulla was significantly reduced compared with the control. After 2 months of the experiment, the studied enzymes were normalized, which indicates the dominance of sanogenic mechanisms in the body. Injury to gonadectomized animals was accompanied by greater inhibition of SOD and catalase activity of the renal cortex and medulla after 1 month of the post-traumatic period. The studied enzymes remained at the same level after 2 months of the experiment. Conclusions. In the conditions of cranioskeletal trauma after 1 month of the post-traumatic period in the renal cortex and medulla there is a violation of the enzymatic link of antioxidant protection, which is manifested by a decrease compared to the control of SOD and catalase activity. In gonadectomized rats, the detected disorders are significantly larger and do not subside after 2 months of the experiment, which indicates the important role of the antioxidant action of estrogen in the pathogenesis of traumatic disease

Keywords

• gonadectomy
• cranioskeletal injury
• superoxide dismutase
• catalase
• kidneys