Nature Communications (Jul 2022)

Mimicked synthetic ribosomal protein complex for benchmarking crosslinking mass spectrometry workflows

  • Manuel Matzinger,
  • Adrian Vasiu,
  • Mathias Madalinski,
  • Fränze Müller,
  • Florian Stanek,
  • Karl Mechtler

DOI
https://doi.org/10.1038/s41467-022-31701-w
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 13

Abstract

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Cross-linking mass spectrometry is widely used to elucidate protein structures and interactions. Here, the authors generate an extensive peptide library to benchmark the most common cross-link search engines with frequently used cross-linking reagents in low and high complex sample systems.