Human induced pluripotent stem cell (iPSC) line (HEBHMUi014-A) derived from a patient with Alzheimer's disease

Mingjing Zhang; Mei Yu; Ruiyun Guo; Yunfei Zhang; Xiaohan Zhang; Jingyi Sun; Jing Zhang; Xia Wang; Jun Ma

Stem Cell Research (Jun 2023)

Human induced pluripotent stem cell (iPSC) line (HEBHMUi014-A) derived from a patient with Alzheimer's disease

Mingjing Zhang,
Mei Yu,
Ruiyun Guo,
Yunfei Zhang,
Xiaohan Zhang,
Jingyi Sun,
Jing Zhang,
Xia Wang,
Jun Ma

Affiliations

Mingjing Zhang: Child Health (Psychological Behavior) Department, Hebei Children's Hospital, Hebei Province 050017, China
Mei Yu: Prenatal Diagnosis Center, Shijiazhuang Obstetrics and Gynecology Hospital, Key Laboratory of Maternal and Fetal Medicine of Hebei Province, Shijiazhuang, Hebei, 050011, China
Ruiyun Guo: Hebei Medical University, University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Yunfei Zhang: Hebei Medical University, University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Emergency Department, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei Province 050000, China
Xiaohan Zhang: Hebei Medical University, University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Jingyi Sun: Hebei Medical University, University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Jing Zhang: Prenatal Diagnosis Center, Shijiazhuang Obstetrics and Gynecology Hospital, Key Laboratory of Maternal and Fetal Medicine of Hebei Province, Shijiazhuang, Hebei, 050011, China; Hebei Medical University, University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Xia Wang: Child Health (Psychological Behavior) Department, Hebei Children's Hospital, Hebei Province 050017, China; Hebei Medical University, University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Corresponding authors at: Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Medical University, Hebei Province 050017, China.
Jun Ma: Hebei Medical University, University of Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Corresponding authors at: Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Medical University, Hebei Province 050017, China.

Journal volume & issue: Vol. 69
p. 103116

Abstract

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The ε4 allele of the lipoprotein E gene (APOE4) is the strongest genetic risk factor associated with sporadic Alzheimer's disease (sAD). While the neuronal cell type-specific function of APOE4 in connection with AD pathology remains understudied. Therefore, we generated an induced pluripotent stem cells (iPSCs) line from a 77-year-old female donor with ApoE4 genetic background. We reprogrammed peripheral blood mononuclear cells (PBMCs) with non-integrative Sendai viral vectors containing reprogramming factors. Established iPSCs showed the capability of pluripotency, three-germ differentiation in vitro with normal karyotype. Hence, the generated iPSC could be a powerful tool to conduct further studies of AD mechanisms.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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