A Peptide‐Derived Aggregation‐Induced Emission Nanobioprobe: Unlocking Selective Detection of Fusobacterium nucleatum and Noninvasive Screening of Colorectal Cancer
Hongyu Liu,
Tengling Wu,
Yunjian Yu,
Youtao Xin,
Hegang Lu,
Shengke Zhao,
Meihui Su,
Lu Ga,
Alideertu Dong,
Mahmoud Elsabahy,
Hui Gao
Affiliations
Hongyu Liu
State Key Laboratory of Separation Membranes and Membrane Processes & Key Laboratory of Hollow Fiber Membrane Materials and Membrane Processes (MOE) & Tianjin Key Laboratory of Hollow Fiber Membrane Materials and Processes School of Materials Science and Engineering Tiangong University Tianjin P. R. China
Tengling Wu
State Key Laboratory of Separation Membranes and Membrane Processes & Key Laboratory of Hollow Fiber Membrane Materials and Membrane Processes (MOE) & Tianjin Key Laboratory of Hollow Fiber Membrane Materials and Processes School of Materials Science and Engineering Tiangong University Tianjin P. R. China
Yunjian Yu
State Key Laboratory of Separation Membranes and Membrane Processes & Key Laboratory of Hollow Fiber Membrane Materials and Membrane Processes (MOE) & Tianjin Key Laboratory of Hollow Fiber Membrane Materials and Processes School of Materials Science and Engineering Tiangong University Tianjin P. R. China
Youtao Xin
State Key Laboratory of Separation Membranes and Membrane Processes & Key Laboratory of Hollow Fiber Membrane Materials and Membrane Processes (MOE) & Tianjin Key Laboratory of Hollow Fiber Membrane Materials and Processes School of Materials Science and Engineering Tiangong University Tianjin P. R. China
Hegang Lu
State Key Laboratory of Separation Membranes and Membrane Processes & Key Laboratory of Hollow Fiber Membrane Materials and Membrane Processes (MOE) & Tianjin Key Laboratory of Hollow Fiber Membrane Materials and Processes School of Materials Science and Engineering Tiangong University Tianjin P. R. China
Shengke Zhao
State Key Laboratory of Separation Membranes and Membrane Processes & Key Laboratory of Hollow Fiber Membrane Materials and Membrane Processes (MOE) & Tianjin Key Laboratory of Hollow Fiber Membrane Materials and Processes School of Materials Science and Engineering Tiangong University Tianjin P. R. China
Meihui Su
State Key Laboratory of Separation Membranes and Membrane Processes & Key Laboratory of Hollow Fiber Membrane Materials and Membrane Processes (MOE) & Tianjin Key Laboratory of Hollow Fiber Membrane Materials and Processes School of Materials Science and Engineering Tiangong University Tianjin P. R. China
Lu Ga
State Key Laboratory of Separation Membranes and Membrane Processes & Key Laboratory of Hollow Fiber Membrane Materials and Membrane Processes (MOE) & Tianjin Key Laboratory of Hollow Fiber Membrane Materials and Processes School of Materials Science and Engineering Tiangong University Tianjin P. R. China
Alideertu Dong
College of Chemistry and Chemical Engineering Engineering Research Center of Dairy Quality and Safety Control Technology Ministry of Education Inner Mongolia University Hohhot P. R. China
Mahmoud Elsabahy
School of Biotechnology Badr University in Cairo Badr City Cairo Egypt
Hui Gao
State Key Laboratory of Separation Membranes and Membrane Processes & Key Laboratory of Hollow Fiber Membrane Materials and Membrane Processes (MOE) & Tianjin Key Laboratory of Hollow Fiber Membrane Materials and Processes School of Materials Science and Engineering Tiangong University Tianjin P. R. China
ABSTRACT Colorectal cancer (CRC) screening and early diagnosis is an effective strategy for reducing CRC mortality. However, the current detection methods involve exorbitant costs and complex procedures, which are inconvenient for large‐scale screening. Given its high prevalence in malignant tissues and feces of CRC patients, Fusobacterium nucleatum (F. nucleatum) has emerged as a crucial biomarker for the early detection of CRC. Herein, we propose an F. nucleatum‐specific recognition strategy for CRC screening and diagnosis. A novel nanobioprobe (AIE‐Pep) with aggregation‐induced emission (AIE) characteristics was synthesized by conjugating a red/near‐infrared (NIR) emissive AIE luminogen (AIEgen) with a FadA‐targeting peptide (ASANWTIQYND). The robust binding affinity between the peptide and FadA on F. nucleatum allows AIE‐Pep NPs to adhere selectively to F. nucleatum, and emits strong red/NIR fluorescence. In the model of the orthotopic CRC, AIE‐Pep NPs can precisely localize F. nucleatum around CRC. Moreover, AIE‐Pep NPs demonstrated a limit of detection (LOD) of 82.97 CFU/mL for F. nucleatum, which could significantly differentiate the feces of CRC mice from those of normal mice. Overall, this study presents a pivotal approach to specifically identifying F. nucleatum and holds immense potential for CRC diagnosis.
