The non-canonical SMC protein SmcHD1 antagonises TAD formation and compartmentalisation on the inactive X chromosome

Michal R. Gdula; Tatyana B. Nesterova; Greta Pintacuda; Jonathan Godwin; Ye Zhan; Hakan Ozadam; Michael McClellan; Daniella Moralli; Felix Krueger; Catherine M. Green; Wolf Reik; Skirmantas Kriaucionis; Edith Heard; Job Dekker; Neil Brockdorff

doi:10.1038/s41467-018-07907-2

Nature Communications (Jan 2019)

The non-canonical SMC protein SmcHD1 antagonises TAD formation and compartmentalisation on the inactive X chromosome

Michal R. Gdula,
Tatyana B. Nesterova,
Greta Pintacuda,
Jonathan Godwin,
Ye Zhan,
Hakan Ozadam,
Michael McClellan,
Daniella Moralli,
Felix Krueger,
Catherine M. Green,
Wolf Reik,
Skirmantas Kriaucionis,
Edith Heard,
Job Dekker,
Neil Brockdorff

Affiliations

Michal R. Gdula: Developmental Epigenetics, Department of Biochemistry, University of Oxford
Tatyana B. Nesterova: Developmental Epigenetics, Department of Biochemistry, University of Oxford
Greta Pintacuda: Developmental Epigenetics, Department of Biochemistry, University of Oxford
Jonathan Godwin: Developmental Epigenetics, Department of Biochemistry, University of Oxford
Ye Zhan: Program in Systems Biology, Department of Medicine, Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Howard Hughes Medical Institute
Hakan Ozadam: Program in Systems Biology, Department of Medicine, Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Howard Hughes Medical Institute
Michael McClellan: Ludwig Cancer Research, Nuffield Department of Medicine, University of Oxford
Daniella Moralli: The Wellcome Centre for Human Genetics, University of Oxford
Felix Krueger: Bioinformatics Group, The Babraham Institute
Catherine M. Green: The Wellcome Centre for Human Genetics, University of Oxford
Wolf Reik: Epigenetics Program, The Babraham Institute
Skirmantas Kriaucionis: Ludwig Cancer Research, Nuffield Department of Medicine, University of Oxford
Edith Heard: Institut Curie, PSL Research University, CNRS UMR3215, INSERM U934, UPMC Paris-Sorbonne
Job Dekker: Program in Systems Biology, Department of Medicine, Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Howard Hughes Medical Institute
Neil Brockdorff: Developmental Epigenetics, Department of Biochemistry, University of Oxford

DOI: https://doi.org/10.1038/s41467-018-07907-2
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 14

Abstract

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The inactive X chromosome (Xi) has an atypical structure, with global loss of TADs, A/B compartments and formation of mega-domains. Here the authors show that the non-canonical SMC family protein, SmcHD1, important for developmental gene silencing on Xi, antagonises TAD formation and compartmentalization on the Xi in a transcription independent way.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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