Different from the McKibben pneumatic muscle actuator, the straight fibers one is made of an elastomeric tube closed at the two ends by two heads that ensure a mechanical and pneumatic seal. High stiffness threads are placed longitudinally into the wall of the tube while external rings are placed at some sections of it to limit the radial expansion of the tube. The inner pressure in the tube causes shortening of the actuator. The working mode of the muscle actuator requires a series of critical repeated contractions and extensions that cause it to rupture. The fatigue life duration of a pneumatic muscle is often lower than traditional pneumatic actuators. The paper presents a procedure for the fatigue life prediction of a straight-fibers muscle based on experimental tests directly carried out with the muscles instead of with specimens of the silicone rubber material which the muscle is made of. The proposed procedure was experimentally validated. Although the procedure is based on fatigue life duration data for silicone rubber, it can be extended to all straight-fibers muscles once the fatigue life duration data of any material considered for the muscles is known.