Influence of plant growth regulators and salicylic acid on the production of some secondary metabolites in callus and cell suspension culture of Satureja sahendica Bornm.

Abstract

The impact of combinations of plant growth regulators (PGRs) on callus culture of Satureja sahendica Bornm. was investigated. In nodal explants, the response of secondary metabolite production to different concentrations of PGRs was analyzed regarding the presence and absence of polyvinylpyrrolidone (PVP). The explants were cultured on MS media in presence of auxins (2,4-dichlorophenoxyacetic acid and naphthylacetic acid) and cytokinins (thidiazuron and kinetin); which were used in equal concentrations of 0.5, 1, and 2 mg l-1. The treatment of 2 mg l-1 2,4-D + 2 mg l-1 Kin (MD3) led to the highest production of total phenolics (4.303 ± 0.449 mg GAE g-1) and flavonoids (24.903 ± 7.016 mg QE g-1). Moreover, the effect of salicylic acid (SA) on the production of secondary metabolites in cell suspension culture of Satureja sahendica was evaluated. The cell suspension culture was established by culturing the nodal-derived friable callus in the liquid medium containing different concentrations of SA (0, 100, 150, 200 µM). An inverse relationship exists between the fresh mass and secondary metabolites contents. In addition, there was a significant difference among concentrations of SA in the production of total phenolics and flavonoid compounds. SA enhances secondary metabolites production and decreases cell fresh mass.

Keywords

• Satureja sahendica
• callus induction
• cell suspension culture
• secondary metabolites
• growth regulators
• salicylic acid