Molecular Study Of Hydatid Disease In Iraqi Goats By using The Mitochondrial Cytochrome C Oxidase Subunit 1 Gene

Abstract

The aim of the present study was to identify and recognize the genotype for cystic Echinococcosis that infects goats (n=19) in Iraq. The hydatid cyst was collected from different parts of the body, i.e. lungs, liver, heart, spleen and peritoneal cavity. The current study was conducted in five different regions of Iraq (Anbar, Baghdad, Saladdin, Karkuk, Babylon during October 2018 to July 2019. The mitochondria DNA was extracted and screened for the presence of (CO1) cytochrome C oxidase subunit 1 gene using polymer chain reactions (PCR). Amplification size was around 450bp. The amplicon was isolated and purified. The isolated CO1 amplicon was sequenced. The CO1 putative sequence was BLAST with available sequence from NCBI. The genetic tree was deduced. The genotype G1 is the most frequently spread strains and considered as a key source for infection in goats of Iraq.

Keywords

• mitochondria dna
• cytochrome c oxidase
• genotype
• goats