Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems

Ashot Papikian; Wanlu Liu; Javier Gallego-Bartolomé; Steven E. Jacobsen

doi:10.1038/s41467-019-08736-7

Nature Communications (Feb 2019)

Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems

Ashot Papikian,
Wanlu Liu,
Javier Gallego-Bartolomé,
Steven E. Jacobsen

Affiliations

Ashot Papikian: Department of Molecular, Cell, and Developmental Biology, University of California
Wanlu Liu: Department of Molecular, Cell, and Developmental Biology, University of California
Javier Gallego-Bartolomé: Department of Molecular, Cell, and Developmental Biology, University of California
Steven E. Jacobsen: Department of Molecular, Cell, and Developmental Biology, University of California

DOI: https://doi.org/10.1038/s41467-019-08736-7
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 11

Abstract

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Few approaches for targeted manipulation of the epigenome are available in plants. Here, the authors adapt the dCas9-SunTag system to engineer targeted gene activation and site-specific manipulation of DNA methylation in Arabidopsis.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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