OncoTargets and Therapy (2019-10-01)

MicroRNA-186-5p Inhibits Proliferation And Metastasis Of Esophageal Cancer By Mediating HOXA9

  • Xu C,
  • Li B,
  • Zhao S,
  • Jin B,
  • Jia R,
  • Ge J,
  • Xu H

Journal volume & issue
Vol. Volume 12
pp. 8905 – 8914

Abstract

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Changqin Xu, Bin Li, Shulei Zhao, Bingjie Jin, Ruzhen Jia, Jian Ge, Hongwei Xu Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, People’s Republic of ChinaCorrespondence: Hongwei XuDepartment of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Huaiyin District, Jinan, Shandong 250021, People’s Republic of China250021 Email [email protected]: MicroRNA (miRNA) is an endogenous, non-coding small RNA that plays a key role in regulating organism biology and pathology. The aim of this study was to investigate the expression characteristics of microRNA-186-5p in esophageal cancer (ECa) and its correlation with clinical progression and prognosis, and to further explore its underlying mechanisms.Methods: Real-time quantitative PCR (qRT-PCR) was used to detect microRNA-186-5p level in 45 pairs of ECa tissue samples and adjacent ones, and to analyze the expression of microRNA-186-5p and clinical progression of ECa and prognosis. The relationship between microRNA-186-5p level in ECa cell lines was further verified by qRT-PCR. Finally, the potential mechanism was explored using luciferase reporter gene assay and cell recovery experiment.Results: QRT-PCR results revealed that the expression of microRNA-186-5p in ECa tissues was remarkably lower than that in adjacent tissues, and the difference was statistically significant. Compared with patients with high expression of microRNA-186-5p, patients with low expression of microRNA-186-5p had higher incidence of pathological stage and lower overall survival rate. Besides, compared with the miR-NC group, the microRNA-186-5p mimics group had a significant decrease in proliferation and metastasis ability of ECa cells. Subsequent qRT-PCR validation in ECa cell lines and tissues indicated a significant increase in HOXA9 expression and a negative correlation with microRNA-186-5p.Conclusion: The expression of microRNA-186-5p was remarkably decreased in ECa, which was remarkably correlated with pathological stage, distant metastasis and poor prognosis of ECa. The results suggested that microRNA-186-5p may inhibit cell proliferation of ECa by regulating HOXA9.Keywords: microRNA-186-5p, HOXA9, esophageal cancer, proliferation, metastasis

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