Improved Salt Tolerance and Metabolomics Analysis of Synechococcus elongatus UTEX 2973 by Overexpressing Mrp Antiporters

Jinyu Cui; Jinyu Cui; Jinyu Cui; Tao Sun; Tao Sun; Shubin Li; Shubin Li; Shubin Li; Yaru Xie; Yaru Xie; Yaru Xie; Xinyu Song; Xinyu Song; Fangzhong Wang; Fangzhong Wang; Lei Chen; Lei Chen; Lei Chen; Weiwen Zhang; Weiwen Zhang; Weiwen Zhang; Weiwen Zhang

doi:10.3389/fbioe.2020.00500

Frontiers in Bioengineering and Biotechnology (May 2020)

Improved Salt Tolerance and Metabolomics Analysis of Synechococcus elongatus UTEX 2973 by Overexpressing Mrp Antiporters

Jinyu Cui,
Jinyu Cui,
Jinyu Cui,
Tao Sun,
Tao Sun,
Shubin Li,
Shubin Li,
Shubin Li,
Yaru Xie,
Yaru Xie,
Yaru Xie,
Xinyu Song,
Xinyu Song,
Fangzhong Wang,
Fangzhong Wang,
Lei Chen,
Lei Chen,
Lei Chen,
Weiwen Zhang,
Weiwen Zhang,
Weiwen Zhang,
Weiwen Zhang

Affiliations

Jinyu Cui: Laboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China
Jinyu Cui: Key Laboratory of Systems Bioengineering, Frontier Science Center for Synthetic Biology, Ministry of Education of China, Tianjin, China
Jinyu Cui: Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, China
Tao Sun: Laboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China
Tao Sun: Center for Biosafety Research and Strategy, Tianjin University, Tianjin, China
Shubin Li: Laboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China
Shubin Li: Key Laboratory of Systems Bioengineering, Frontier Science Center for Synthetic Biology, Ministry of Education of China, Tianjin, China
Shubin Li: Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, China
Yaru Xie: Laboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China
Yaru Xie: Key Laboratory of Systems Bioengineering, Frontier Science Center for Synthetic Biology, Ministry of Education of China, Tianjin, China
Yaru Xie: Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, China
Xinyu Song: Laboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China
Xinyu Song: Center for Biosafety Research and Strategy, Tianjin University, Tianjin, China
Fangzhong Wang: Laboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China
Fangzhong Wang: Center for Biosafety Research and Strategy, Tianjin University, Tianjin, China
Lei Chen: Laboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China
Lei Chen: Key Laboratory of Systems Bioengineering, Frontier Science Center for Synthetic Biology, Ministry of Education of China, Tianjin, China
Lei Chen: Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, China
Weiwen Zhang: Laboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China
Weiwen Zhang: Key Laboratory of Systems Bioengineering, Frontier Science Center for Synthetic Biology, Ministry of Education of China, Tianjin, China
Weiwen Zhang: Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, China
Weiwen Zhang: Center for Biosafety Research and Strategy, Tianjin University, Tianjin, China

DOI: https://doi.org/10.3389/fbioe.2020.00500
Journal volume & issue: Vol. 8

Abstract

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The fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 (Syn2973) is a promising candidate for photosynthetic microbial factory. Seawater utilization is necessary for large-scale cultivation of Syn2973 in the future. However, Syn2973 is sensitive to salt stress, making it necessary to improve its salt tolerance. In this study, 21 exogenous putative transporters were individually overexpressed in Syn2973 to evaluate their effects on salt tolerance. The results showed the overexpression of three Mrp antiporters significantly improved the salt tolerance of Syn2973. Notably, overexpressing the Mrp antiporter from Synechococcus sp. PCC 7002 improved cell growth by 57.7% under 0.4 M NaCl condition. In addition, the metabolomics and biomass composition analyses revealed the possible mechanisms against salt stress in both Syn2973 and the genetically engineered strain. The study provides important engineering strategies to improve salt tolerance of Syn2973 and is valuable for understanding mechanisms of salt tolerance in cyanobacteria.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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