Prognostic relevance of tumor-infiltrating CD4<sup>+</sup> cells and total metabolic tumor volume-based risk stratification in diffuse large B-cell lymphoma
Daisuke Ikeda,
Mitsuaki Oura,
Atsushi Uehara,
Rikako Tabata,
Kentaro Narita,
Masami Takeuchi,
Youichi Machida,
Kosei Matsue
Affiliations
Daisuke Ikeda
Division of Hematology/Oncology, Department of Medicine, Kameda Medical Center, Chiba
Mitsuaki Oura
Division of Hematology/Oncology, Department of Medicine, Kameda Medical Center, Chiba
Atsushi Uehara
Division of Hematology/Oncology, Department of Medicine, Kameda Medical Center, Chiba
Rikako Tabata
Division of Hematology/Oncology, Department of Medicine, Kameda Medical Center, Chiba
Kentaro Narita
Division of Hematology/Oncology, Department of Medicine, Kameda Medical Center, Chiba
Masami Takeuchi
Division of Hematology/Oncology, Department of Medicine, Kameda Medical Center, Chiba
Youichi Machida
Department of Radiology, Kameda Medical Center, Chiba
Kosei Matsue
Division of Hematology/Oncology, Department of Medicine, Kameda Medical Center, Chiba
To elucidate the relationship between pre-treatment radiomic parameters and the proportions of various tumour-infiltrating (TI) cells, we retrospectively analysed the association of total metabolic tumour volume (TMTV) and TI cells on biopsied tumour lesions in 171 patients with newly diagnosed diffuse large B-cell lymphoma (DLBCL). The surface markers of TI cells were analysed by multicolour flow cytometry using a dissected single-cell suspension. In examining the correlation between TI cells and PET-derived parameters (SUVmax, TMTV, and total lesion glycolysis), intratumoural cell types minimally influenced the results, except for a weak negative correlation between CD4+ cells and SUVmax (R=-0.16, P=0.045). Even for the lesion fluorodeoxyglucose uptake at the biopsied site, CD19+ cells (indicative of malignant burden) showed only a weak correlation with the highest SUV (R=0.21, P=0.009), whereas CD3+ (R=-0.25, P=0.002) and CD4+ cells (R=-0.29, P
