Characterization of difference in muscle volatile compounds between triploid and diploid crucian carp

Abstract

The present study was conducted to investigate the volatile compounds profile of raw muscle between diploid (2n = 100) and triploid crucian carp (produced by hybridization from mating diploid crucian carp to allotetraploid, 3n = 150) cultured under the same conditions. Volatile compounds of crucian carp between ploidy were qualitatively analyzed by headspace solid-phase microextraction and gas chromatography-tandem mass spectrometry (HS-SPME-GC/MS). A total of 52 volatiles were identified in the two groups of crucian carp including 5 alcohols, 5 aromatics, 8 aldehydes, 1 ketone, 10 alkanes, 19 alkenes and 4 esters. No significant difference was observed in the total relative concentration between the two groups (P > 0.05). Based on the data of odor-active value (OAV ≥ 1), 10 odor-active compounds may mainly contribute to the overall odor, including 1-nonanol, 1-octen-3-ol, hexanal, decanal, octanal, 1-nonanal, p-cymene, styrene, limonene and tridecane. Triploid crucian carp had more odor-active compounds and higher total OAV value than that of diploid (P < 0.05). With the results of partial least squares discrimination analysis (PLS-DA) in total volatiles and odor-active compounds, the overall odor of triploid and diploid crucian carp was obviously different.

Keywords

• Hybridization
• Triploid
• Crucian carp
• Volatile compounds
• Odor-active value