Biological Properties of Low-Toxicity PLGA and PLGA/PHB Fibrous Nanocomposite Implants for Osseous Tissue Regeneration. Part I: Evaluation of Potential Biotoxicity
Izabella Krucińska,
Bogusława Żywicka,
Agnieszka Komisarczyk,
Maria Szymonowicz,
Stanisława Kowalska,
Ewa Zaczyńska,
Marcin Struszczyk,
Anna Czarny,
Piotr Jadczyk,
Barbara Umińska-Wasiluk,
Zbigniew Rybak,
Marek Kowalczuk
Affiliations
Izabella Krucińska
Department of Material and Commodity Sciences and Textile Metrology, Technical University of Lodz, Zeromskiego 116, 90-924 Lodz, Poland
Bogusława Żywicka
Department of Experimental Surgery and Biomaterials Research, Wroclaw Medical University, Pasteura 1, 50-367 Wroclaw, Poland
Agnieszka Komisarczyk
Department of Material and Commodity Sciences and Textile Metrology, Technical University of Lodz, Zeromskiego 116, 90-924 Lodz, Poland
Maria Szymonowicz
Department of Experimental Surgery and Biomaterials Research, Wroclaw Medical University, Pasteura 1, 50-367 Wroclaw, Poland
Stanisława Kowalska
Department of Material and Commodity Sciences and Textile Metrology, Technical University of Lodz, Zeromskiego 116, 90-924 Lodz, Poland
Ewa Zaczyńska
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, RudollfaWeigla 12, 53-114 Wroclaw, Poland
Marcin Struszczyk
Department of Material and Commodity Sciences and Textile Metrology, Technical University of Lodz, Zeromskiego 116, 90-924 Lodz, Poland
Anna Czarny
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, RudollfaWeigla 12, 53-114 Wroclaw, Poland
Piotr Jadczyk
Department of Sanitary Biology and Ecotechnics, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland
Barbara Umińska-Wasiluk
Department of Sanitary Biology and Ecotechnics, Wroclaw University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland
Zbigniew Rybak
Department of Experimental Surgery and Biomaterials Research, Wroclaw Medical University, Pasteura 1, 50-367 Wroclaw, Poland
Marek Kowalczuk
Centre of Polymer and Carbon Materials of the Polish Academy of Sciences, MariiSkłodowskiej-Curie 34, 41-819 Zabrze, Poland
In response to the demand for new implant materials characterized by high biocompatibility and bioresorption, two prototypes of fibrous nanocomposite implants for osseous tissue regeneration made of a newly developed blend of poly(l-lactide-co-glycolide) (PLGA) and syntheticpoly([R,S]-3-hydroxybutyrate), PLGA/PHB, have been developed and fabricated. Afibre-forming copolymer of glycolide and l-lactide (PLGA) was obtained by a unique method of synthesis carried out in blocksusing Zr(AcAc)4 as an initiator. The prototypes of the implants are composed of three layers of PLGA or PLGA/PHB, nonwoven fabrics with a pore structure designed to provide the best conditions for the cell proliferation. The bioactivity of the proposed implants has been imparted by introducing a hydroxyapatite material and IGF1, a growth factor. The developed prototypes of implants have been subjected to a set of in vitro and in vivobiocompatibility tests: in vitro cytotoxic effect, in vitro genotoxicity and systemic toxicity. Rabbitsshowed no signs of negative reactionafter implantation of the experimental implant prototypes.
