Inhibition of Bacterial Adhesion and Biofilm Formation by Seed-Derived Ethanol Extracts from <i>Persea americana</i> Mill
Silvia del Carmen Molina Bertrán,
Lianet Monzote,
Davie Cappoen,
Julio Cesar Escalona Arranz,
Mario Juan Gordillo Pérez,
Annarli O. Rodríguez-Ferreiro,
Idelsy Chill Nuñez,
Claudina Pérez Novo,
Daniel Méndez,
Paul Cos,
Gabriel Llauradó Maury
Affiliations
Silvia del Carmen Molina Bertrán
Pharmacy Department, Faculty of Natural and Exact Sciences, University of Oriente, Santiago de Cuba 90500, Cuba
Lianet Monzote
Department of Parasitology, Institute of Tropical Medicine “Pedro Kourí”, Havana 11400, Cuba
Davie Cappoen
Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, 2610 Antwerp, Belgium
Julio Cesar Escalona Arranz
Pharmacy Department, Faculty of Natural and Exact Sciences, University of Oriente, Santiago de Cuba 90500, Cuba
Mario Juan Gordillo Pérez
Biology Department, Faculty of Natural and Exact Sciences, University of Oriente, Santiago de Cuba 90500, Cuba
Annarli O. Rodríguez-Ferreiro
Department of Biomedical Engineering, Faculty of Telecom, Informatics and Biomedical Engineering, University of Oriente, Santiago de Cuba 90500, Cuba
Idelsy Chill Nuñez
Pharmacy Department, Faculty of Natural and Exact Sciences, University of Oriente, Santiago de Cuba 90500, Cuba
Claudina Pérez Novo
Laboratory for Protein Chemistry, Proteomics and Epigenetic Signaling, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, 2610 Antwerp, Belgium
Daniel Méndez
Chemistry Department, Faculty of Applied Sciences, University of Camagüey, Carretera de Circunvalación Km 512, Camagüey 74650, Cuba
Paul Cos
Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, 2610 Antwerp, Belgium
Gabriel Llauradó Maury
Center of Studies for Industrial Biotechnology (CEBI), University of Oriente, Santiago de Cuba 90500, Cuba
The increase in antibiotic resistance demands innovative strategies to combat microorganisms. The current study evaluated the antibacterial and antivirulence effects of ethanol extracts from Persea americana seeds obtained by the Soxhlet (SE) and maceration (MaE) methods. The UHPLC-DAD-QTOF analysis showed mainly the presence of polyphenols and neolignan. Ethanol extracts were not cytotoxic to mammalian cells (CC50 > 500 µg/mL) and displayed a moderate antibacterial activity against Pseudomonas aeruginosa (IC50 = 87 and 187 µg/mL) and Staphylococcus aureus (IC50 = 144 and 159 µg/mL). Interestingly, no antibacterial activity was found against Escherichia coli. SE and MaE extracts were also able to significantly reduce the bacterial adhesion to A549 lung epithelial cells. Additionally, both extracts inhibited the biofilm growth at 24 h and facilitated the release of internal cell components in P. aeruginosa, which might be associated with cell membrane destabilization. Real-time PCR and agarose electrophoresis gel analysis indicated that avocado seed ethanol extracts (64 µg/mL) downregulated virulence-related factors such as mexT and lasA genes. Our results support the potential of bioproducts from P. americana seeds as anti-adhesive and anti-biofilm agents.
