Comparative Serological and Random Amplified Polymorphic DNA Typing for Bordetella avium Isolates in China

Abstract

To study the similarity among Bordetella avium isolates in China, antigens and diagnostic antiserum of 22 B. avium isolates were prepared for serotyping, and a set of 20 commercially available primers was screened out to identify suitable primers for random amplified polymorphic DNA fingerprinting (RAPD) analysis in this study. Twenty-two B. avium isolates were divided into two serovars (A and B) based on their reaction in the plate-agglutination test. Four primers R1, R2, R4 and R10 resulted in informative fingerprints and were used to evaluate the B. avium isolates. Based on their RAPD patterns, a dendrogram allowed the separation of the B. avium isolates into six genetic similarity clusters. However, no direct correlation was observed between serotypes and RAPD typing among the isolates.

Keywords

• Bordetella avium
• Plate-agglutination test
• RAPD typing
• Serotyping