DosR’s multifaceted role on Mycobacterium bovis BCG revealed through multi-omics

Yingying Cui; Guanghui Dang; Hui Wang; Yiyi Tang; Mingyue Lv; Siguo Liu; Ningning Song; Ningning Song; Ningning Song

doi:10.3389/fcimb.2023.1292864

Frontiers in Cellular and Infection Microbiology (Nov 2023)

DosR’s multifaceted role on Mycobacterium bovis BCG revealed through multi-omics

Yingying Cui,
Guanghui Dang,
Hui Wang,
Yiyi Tang,
Mingyue Lv,
Siguo Liu,
Ningning Song,
Ningning Song,
Ningning Song

Affiliations

Yingying Cui: State Key Laboratory for Animal Disease Control and Prevention, Division of Bacterial Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
Guanghui Dang: State Key Laboratory for Animal Disease Control and Prevention, Division of Bacterial Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
Hui Wang: State Key Laboratory for Animal Disease Control and Prevention, Division of Bacterial Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
Yiyi Tang: State Key Laboratory for Animal Disease Control and Prevention, Division of Bacterial Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
Mingyue Lv: State Key Laboratory for Animal Disease Control and Prevention, Division of Bacterial Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
Siguo Liu: State Key Laboratory for Animal Disease Control and Prevention, Division of Bacterial Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
Ningning Song: State Key Laboratory for Animal Disease Control and Prevention, Division of Bacterial Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China
Ningning Song: School of Life Science and Technology, Weifang Medical University, Weifang, China
Ningning Song: Weifang Key Laboratory of Respiratory Tract Pathogens and Drug Therapy, Weifang, China

DOI: https://doi.org/10.3389/fcimb.2023.1292864
Journal volume & issue: Vol. 13

Abstract

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Mycobacterium tuberculosis (Mtb) is an intracellular bacterium that causes a highly contagious and potentially lethal tuberculosis (TB) in humans. It can maintain a dormant TB infection within the host. DosR (dormancy survival regulator) (Rv3133c) has been recognized as one of the key transcriptional proteins regulating bacterial dormancy and participating in various metabolic processes. In this study, we extensively investigate the still not well-comprehended role and mechanism of DosR in Mycobacterium bovis (M. bovis) Bacillus Calmette-Guérin (BCG) through a combined omics analysis. Our study finds that deleting DosR significantly affects the transcriptional levels of 104 genes and 179 proteins. Targeted metabolomics data for amino acids indicate that DosR knockout significantly upregulates L-Aspartic acid and serine synthesis, while downregulating seven other amino acids, including L-histidine and lysine. This suggests that DosR regulates amino acid synthesis and metabolism. Taken together, these findings provide molecular and metabolic bases for DosR effects, suggesting that DosR may be a novel regulatory target.

Published in Frontiers in Cellular and Infection Microbiology

ISSN: 2235-2988 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/Cellular-and-Infection-Microbiology

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