Structural basis for DNA unwinding at forked dsDNA by two coordinating Pif1 helicases

Nannan Su; Alicia K. Byrd; Sakshibeedu R. Bharath; Olivia Yang; Yu Jia; Xuhua Tang; Taekjip Ha; Kevin D. Raney; Haiwei Song

doi:10.1038/s41467-019-13414-9

Nature Communications (Nov 2019)

Structural basis for DNA unwinding at forked dsDNA by two coordinating Pif1 helicases

Nannan Su,
Alicia K. Byrd,
Sakshibeedu R. Bharath,
Olivia Yang,
Yu Jia,
Xuhua Tang,
Taekjip Ha,
Kevin D. Raney,
Haiwei Song

Affiliations

Nannan Su: Life Sciences Institute, Zhejiang University
Alicia K. Byrd: Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences
Sakshibeedu R. Bharath: Institute of Molecular and Cell Biology
Olivia Yang: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University
Yu Jia: Life Sciences Institute, Zhejiang University
Xuhua Tang: Institute of Molecular and Cell Biology
Taekjip Ha: Department of Biophysics and Biophysical Chemistry, Johns Hopkins University
Kevin D. Raney: Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences
Haiwei Song: Life Sciences Institute, Zhejiang University

DOI: https://doi.org/10.1038/s41467-019-13414-9
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 11

Abstract

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Pif1 plays multiple roles in maintaining genome stability and preferentially unwinds forked dsDNA. Here the authors solve the structure of Bacteroides sp Pif1 (BaPif1) in complex with a symmetrical double forked dsDNA and provide unprecedented insights into forked dsDNA unwinding by BaPif1.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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