miR-132 can inhibit glioma cells invasion and migration by target MMP16 in vitro

Abstract

Hangzhou Wang,1,2,* Xue-tao Li,1,* Chun Wu,1 Zhi-wu Wu,1 Yan-yan Li,1 Tian-quan Yang,1 Gui-lin Chen,1 Xue-shun Xie,1 Yu-lun Huang,1 Zi-wei Du,1 You-xin Zhou1 1Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, People’s Republic of China; 2Department of Neurosurgery, Children’s Hospital Affiliated to Soochow University, Suzhou, Jiangsu, People’s Republic of China *These authors contributed equally to this work Abstract: Gliomas are the most common malignant primary brain tumors, and new clinical biomarkers and therapeutic targets are imminently required. MicroRNAs (miRNAs) are a novel class of small non-coding RNAs (~22nt) involved in the regulation of various biological processes. Here, by using real-time polymerase chain reaction, miRNA-132 was found to be significantly deregulated in glioma tissues. Based on the prediction of the target genes of miR-132, we hypothesized that there is a significant association between miR-132 and matrix metalloproteinase (MMP) 16 (MT3-MMP), a protein of the MMP family. We showed that the up-expression of miR-132 inhibited cell migration and invasion in the human glioma cell lines A172, SHG44, and U87. Furthermore, the overexpression of miR-132 reduced the expression of MMP16 in A172, SHG44, and U87 cells. Taken together, our study suggested that miR-132 affects glioma cell migration and invasion by MMP16 and implicates miR-132 as a metastasis-inhibiting miRNA in gliomas. Keywords: gliocytoma, microRNA, MT3-MMP, invasions