Effect of alkaline protease produced from fish waste as substrate by Bacillus clausii on destaining of blood stained fabric

Abstract

Alkaline protease or peptidases are the largest groups of enzymes in the biological industry with a variety of application in manufacturing units used in the process of substrate stabilization, dehairing, diagnosis, extraction, food production, destaining,etc., where the pH of the environmental conditions remain above neutral pH. Because of these widerapplications of alkaline proteases in industries, their demand is increas-ing to compete with their chemical counterpart. An alkaline tolerant bacterial strain Bacillusclausiiwasisolatedfromfishwasteandusedformassproduction of alkaline protease using fish waste homogenate as media. Preliminary study on optimization of conditions for the mass production carried out. The optimum temperature for produc-tion ranges between 25°C and 35°C and pH determined as 9. The mass production of extracellular alkaline protease carried out using mobilized and immobilized cells of B. clausiiat optimized condition using production media, the mixture of production media and fish waste homogenate and innutrient broth. The recorded results showed that the maximum enzyme productionobtainedwith immobilized cells in nutrient broth media and followed by fish waste homogenate media of 8900U/ml and 8600U/ml.Purification protease enzyme yielded 0.35 g/mlfr o m t h e productionm e d i a. Blood-stained cloth treated with immobilized enzymeremoved the stain completely com-pared to treatment with non-immobilized enzyme and commercially used detergent. So,the current study suggests theusageofmicrobialalkalineproteaseinhouseholddetergentstoreplacethe usage of synthetic detergents and save the environment from chemicalpollutants.

Keywords

• alkaline protease
• destaining
• fish waste media
• immobilization
• mass production
• optimization