Nature Communications (Mar 2018)
Engineering enhanced cellobiohydrolase activity
- Larry E. Taylor,
- Brandon C. Knott,
- John O. Baker,
- P. Markus Alahuhta,
- Sarah E. Hobdey,
- Jeffrey G. Linger,
- Vladimir V. Lunin,
- Antonella Amore,
- Venkataramanan Subramanian,
- Kara Podkaminer,
- Qi Xu,
- Todd A. VanderWall,
- Logan A. Schuster,
- Yogesh B. Chaudhari,
- William S. Adney,
- Michael F. Crowley,
- Michael E. Himmel,
- Stephen R. Decker,
- Gregg T. Beckham
Affiliations
- Larry E. Taylor
- Biosciences Center, National Renewable Energy Laboratory
- Brandon C. Knott
- Biosciences Center, National Renewable Energy Laboratory
- John O. Baker
- Biosciences Center, National Renewable Energy Laboratory
- P. Markus Alahuhta
- Biosciences Center, National Renewable Energy Laboratory
- Sarah E. Hobdey
- Biosciences Center, National Renewable Energy Laboratory
- Jeffrey G. Linger
- National Bioenergy Center, National Renewable Energy Laboratory
- Vladimir V. Lunin
- Biosciences Center, National Renewable Energy Laboratory
- Antonella Amore
- Biosciences Center, National Renewable Energy Laboratory
- Venkataramanan Subramanian
- Biosciences Center, National Renewable Energy Laboratory
- Kara Podkaminer
- Biosciences Center, National Renewable Energy Laboratory
- Qi Xu
- Biosciences Center, National Renewable Energy Laboratory
- Todd A. VanderWall
- Biosciences Center, National Renewable Energy Laboratory
- Logan A. Schuster
- Biosciences Center, National Renewable Energy Laboratory
- Yogesh B. Chaudhari
- Biosciences Center, National Renewable Energy Laboratory
- William S. Adney
- Biosciences Center, National Renewable Energy Laboratory
- Michael F. Crowley
- Biosciences Center, National Renewable Energy Laboratory
- Michael E. Himmel
- Biosciences Center, National Renewable Energy Laboratory
- Stephen R. Decker
- Biosciences Center, National Renewable Energy Laboratory
- Gregg T. Beckham
- National Bioenergy Center, National Renewable Energy Laboratory
- DOI
- https://doi.org/10.1038/s41467-018-03501-8
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 10
Abstract
Cellobiohydrolases (CBHs) are critical for natural and industrial biomass degradation but their structure–activity relationships are not fully understood. Here, the authors present the biochemical and structural characterization of two CBHs, identifying protein regions that confer enhanced CBH activity.
