Open syntaxin overcomes exocytosis defects of diverse mutants in C. elegans

Chi-Wei Tien; Bin Yu; Mengjia Huang; Karolina P. Stepien; Kyoko Sugita; Xiaoyu Xie; Liping Han; Philippe P. Monnier; Mei Zhen; Josep Rizo; Shangbang Gao; Shuzo Sugita

doi:10.1038/s41467-020-19178-x

Nature Communications (Nov 2020)

Open syntaxin overcomes exocytosis defects of diverse mutants in C. elegans

Chi-Wei Tien,
Bin Yu,
Mengjia Huang,
Karolina P. Stepien,
Kyoko Sugita,
Xiaoyu Xie,
Liping Han,
Philippe P. Monnier,
Mei Zhen,
Josep Rizo,
Shangbang Gao,
Shuzo Sugita

Affiliations

Chi-Wei Tien: Division of Fundamental Neurobiology, Krembil Brain Institute, University Health Network
Bin Yu: Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology
Mengjia Huang: Division of Fundamental Neurobiology, Krembil Brain Institute, University Health Network
Karolina P. Stepien: Department of Biophysics, University of Texas Southwestern Medical Center
Kyoko Sugita: Division of Genetics and Development, Krembil Brain Institute, University Health Network
Xiaoyu Xie: Division of Fundamental Neurobiology, Krembil Brain Institute, University Health Network
Liping Han: Department of Anesthesiology, Dalian Medical University
Philippe P. Monnier: Faculty of Medicine, Department of Physiology, University of Toronto
Mei Zhen: Faculty of Medicine, Department of Physiology, University of Toronto
Josep Rizo: Department of Biophysics, University of Texas Southwestern Medical Center
Shangbang Gao: Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology
Shuzo Sugita: Division of Fundamental Neurobiology, Krembil Brain Institute, University Health Network

DOI: https://doi.org/10.1038/s41467-020-19178-x
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 18

Abstract

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Opening of the UNC-64/syntaxin closed conformation by UNC-13/Munc13 to form the neuronal SNARE complex is critical for neurotransmitter release. Here the authors show that facilitating the opening of syntaxin enhances exocytosis not only in unc-13 nulls as well as in diverse C. elegans mutants.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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