Inhalable Microparticles Embedding Calcium Phosphate Nanoparticles for Heart Targeting: The Formulation Experimental Design
Eride Quarta,
Fabio Sonvico,
Ruggero Bettini,
Claudio De Luca,
Alessandro Dotti,
Daniele Catalucci,
Michele Iafisco,
Lorenzo Degli Esposti,
Gaia Colombo,
Giovanna Trevisi,
Dimitrios M. Rekkas,
Alessandra Rossi,
Tin Wui Wong,
Francesca Buttini,
Paolo Colombo
Affiliations
Eride Quarta
Food and Drug Department, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, Italy
Fabio Sonvico
Food and Drug Department, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, Italy
Ruggero Bettini
Food and Drug Department, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, Italy
Claudio De Luca
Fin-Ceramica Faenza SPA, Via Granarolo 177/3, 48018 Faenza, Italy
Alessandro Dotti
Fin-Ceramica Faenza SPA, Via Granarolo 177/3, 48018 Faenza, Italy
Daniele Catalucci
IRCCS Humanitas Research Hospital, 20089 Rozzano, Italy
Michele Iafisco
Institute of Science and Technology for Ceramics (ISTEC), National Research Council (CNR), Via Granarolo 64, 48018 Faenza, Italy
Lorenzo Degli Esposti
Institute of Science and Technology for Ceramics (ISTEC), National Research Council (CNR), Via Granarolo 64, 48018 Faenza, Italy
Gaia Colombo
Department of Life Sciences and Biotechnology, University of Ferrara, Via Fossato di Mortara 17/19, 44121 Ferrara, Italy
Giovanna Trevisi
Institute of Materials for Electronics and Magnetism (IMEM), National Research Council (CNR), Parco Area delle Scienze 37/A, 43124 Parma, Italy
Dimitrios M. Rekkas
Department of Pharmacy, National and Kapodistrian University of Athens, 15784 Zografou, Athens, Greece
Alessandra Rossi
Food and Drug Department, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, Italy
Tin Wui Wong
Non-Destructive Biomedical and Pharmaceutical Research Centre, Smart Manufacturing Research Institute, Universiti Teknologi MARA, Puncak Alam 42300, Selangor, Malaysia
Francesca Buttini
Food and Drug Department, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, Italy
Paolo Colombo
Food and Drug Department, University of Parma, Parco Area delle Scienze 27/A, 43124 Parma, Italy
Inhalation of Calcium Phosphate nanoparticles (CaPs) has recently unmasked the potential of this nanomedicine for a respiratory lung-to-heart drug delivery targeting the myocardial cells. In this work, we investigated the development of a novel highly respirable dry powder embedding crystalline CaPs. Mannitol was selected as water soluble matrix excipient for constructing respirable dry microparticles by spray drying technique. A Quality by Design approach was applied for understanding the effect of the feed composition and spraying feed rate on typical quality attributes of inhalation powders. The in vitro aerodynamic behaviour of powders was evaluated using a medium resistance device. The inner structure and morphology of generated microparticles were also studied. The 1:4 ratio of CaPs/mannitol led to the generation of hollow microparticles, with the best aerodynamic performance. After microparticle dissolution, the released nanoparticles kept their original size.
