The neoepitope of the complement C5b-9 Membrane Attack Complex is formed by proximity of adjacent ancillary regions of C9

Charles Bayly-Jones; Bill H. T. Ho; Corinna Lau; Eleanor W. W. Leung; Laura D’Andrea; Christopher J. Lupton; Susan M. Ekkel; Hariprasad Venugopal; James C. Whisstock; Tom E. Mollnes; Bradley A. Spicer; Michelle A. Dunstone

doi:10.1038/s42003-023-04431-y

Communications Biology (Jan 2023)

The neoepitope of the complement C5b-9 Membrane Attack Complex is formed by proximity of adjacent ancillary regions of C9

Charles Bayly-Jones,
Bill H. T. Ho,
Corinna Lau,
Eleanor W. W. Leung,
Laura D’Andrea,
Christopher J. Lupton,
Susan M. Ekkel,
Hariprasad Venugopal,
James C. Whisstock,
Tom E. Mollnes,
Bradley A. Spicer,
Michelle A. Dunstone

Affiliations

Charles Bayly-Jones: Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University
Bill H. T. Ho: Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University
Corinna Lau: Research laboratory, Nordland Hospital Trust
Eleanor W. W. Leung: Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University
Laura D’Andrea: Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University
Christopher J. Lupton: Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University
Susan M. Ekkel: Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University
Hariprasad Venugopal: Ramaciotti Centre for Cryo-Electron Microscopy, Monash University
James C. Whisstock: Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University
Tom E. Mollnes: Research laboratory, Nordland Hospital Trust
Bradley A. Spicer: Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University
Michelle A. Dunstone: Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University

DOI: https://doi.org/10.1038/s42003-023-04431-y
Journal volume & issue: Vol. 6, no. 1
pp. 1 – 11

Abstract

Read online

The cryo-EM structure of monoclonal antibody aE11 bound to polyC9 reveals that the binding site within the Membrane Attack Complex is a quaternary discontinuous epitope formed by two separate surfaces of the oligomeric C9 periphery.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

About the journal