Frontiers in Chemistry (Oct 2022)

Detection of abemaciclib, an anti-breast cancer agent, using a new electrochemical DNA biosensor

  • Zimeng Lei,
  • Merim Alwan,
  • Hassan Thoulfikar A. Alamir,
  • Hussein Humedy Chlib Alkaaby,
  • Sinan Subhi Farhan,
  • Sura A. Awadh,
  • Usama S. Altimari,
  • Hawra’a Fadhel Abbas Al-Baghdady,
  • Athmar Ali Kadhim,
  • Maytham T. Qasim,
  • Ali Hussein Adhab,
  • Abuzar Nekuei

DOI
https://doi.org/10.3389/fchem.2022.980162
Journal volume & issue
Vol. 10

Abstract

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Detection of DNA molecules and possible chemotherapy-induced changes in its structure has been the goal of researchers using rapid, sensitive and inexpensive approaches. Therefore, the aim of this study was to fabricate a new electrochemical DNA biosensor using pencil graphite electrodes modified with polypyrrole/Ce doped hexagonal nickel oxide nanodisks or PP/Ce-doped H-NiO-ND composites for determination of Abemaciclib (AMC) and ds-DNA molecules. The DNA biosensor was prepared by immobilizing ds-DNA on the surface of PP/Ce-doped H-NiO-ND/PGE. Differential pulse voltammetry (DPV) was used to electrochemically detect AMC. The results elucidate the extremely high sensitivity of the ds-DNA/PP/Ce-doped H-NiO-ND/PGE biosensor to AMC, with a narrow detection limit of 2.7 nM and a lengthy linear range of 0.01–600.0 μM. The admirable performance of as-fabricated biosensor could be related to the active reaction sites and the unique electrochemical response related to the nanocomposites by enhancing ds-DNA stabilization and accelerating electron transfer on the surface of electrode.

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