Effect of Mytomycin C Treatment on Feeder Cells and the Proliferation of Cultured Chicken Primordial Germ Cells Isolated from Embryonic Blood

Abstract

Chicken primordial germ cells isolated from embryonic blood were cultured in vitro on feeder cells derived from the gonads of 7-day incubated embryos. Primordial germ cells were proliferated about 13 times during the 4 day culture period on the feeder cells without mytomycin C treatment. When the feeder cells were treated with mytomycin C for 1 hour, primordial germ cells proliferated about 7 times by day 3 of culture and then decreased in number. When the feeder cells were treated with mytomycin C for 2-3 hours, almost no proliferation of primordial germ cells was observed. These results suggest that the interaction between primordial germ cells and feeder cells for proliferating primordial germ cells was suppressed by treating the feeder cells with mytomycin C.

Keywords

• chicken
• primordial germ cell
• culture
• feeder cell
• mytomycin c